Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3611-3615.doi: 10.3969/j.issn.1673-8225.2011.20.001

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Construction of vascularization artificial bone in vitro and ectopic osteogenesis in vivo

Guo Ying1, Ma Wei-dong2, Chen Xiao-dong1, Qu Zhe2   

  1. 1Department of Prosthodontics, 2Department of Implantology, Dalian Stomatological Hospital, Dalian  116021, Liaoning Province, China
  • Received:2010-11-26 Revised:2011-01-15 Online:2011-05-14 Published:2011-05-14
  • Contact: Qu Zhe, Doctor, Associate professor, Department of Implantology, Dalian Stomatological Hospital, Dalian 116021, Liaoning Province, China quzhekq@hotmail. com
  • About author:Guo Ying★, Master, Attending physician, Department of Prosthodontics, Dalian Stomatological Hospital, Dalian 116021, Liaoning Province, China guohining@hotmail. com
  • Supported by:

    the Science and Technology Development Plan of Education Department of Liaoning Province, No. 2008027*

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Abstract:

BACKGROUND: Vascularization plays an important role in bone formation and remodeling, however, the oxygen and nutrition are insufficient for large tissue block; therefore, it is necessary to solve the problem of vascularization in tissue engineering field. 
OBJECTIVE: To investigate the method of constructing vascularization artificial bone in vitro and ectopic osteogenesis in vivo.
METHODS: Rat bone marrow mesenchymal stem cells (BMSCs) and kidney vascular endothelial cells were isolated and cultured, and osteogenesis ability of BMSCs in different co-culture models (co-culture with or without direct contact) were analysed by measuring the quantity of protein and the activity of alkaline phosphatase and osteocalcin. By establishing the three-dimensional co-culture model of rat BMSCs and kidney vascular endothelial cells, vascularized artificial bone was constructed, which was implanted into the muscle of rats. The angiopoiesis and osteogenetic ability of the implants were observed by soft X-ray examination and hematoxylin-eosin staining.
RESULTS AND CONCLUSION: There was good cell compatibility when rat BMSCs and kidney vascular endothelial cells were co-cultured. The ALP activity and osteocalcin was decreased in the simple culture groups, but increased in the direct and indirect co-culture groups. Statistical analysis showed that the osteogenetic ability of BMSCs co-cultured group was higher than that of simple cultured (P < 0.05). The in vivo experiment manifested that the intensity of soft X-ray and the quantity of vascular and new formed bone in the vascularized artificial bone group were higher than those in the control group (P < 0.05). The osteogenetic ability of BMSCs can be regulated by cytokines and cell membrane proteins when co-cultured with endothelial cells. Compared with traditional artificial bone, vascularized artificial bones has lots of advantages, such as accelerating the differentiation and proliferation of BMSCs, increasing local blood circulation and survive ratio, as well as accelerating osteogenesis and having more powerful anti-infect ability.

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