Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3555-3558.doi: 10.3969/j.issn.1673-8225.2011.19.032

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Stem cell characteristics of human amniotic epithelial cells

Jin Ling1, Chen Jian2, Wu Jing3, Xu Jin-tang3, Zhou Qing2, Liu Xiao-yong2   

  1. 1People’s Hospital of Baoan District, Shenzhen  518101, Guangdong Province, China
    2Department of Ophthalmology, First Affiliated Hospital of Jinan University, Guangzhou  510630, Guangdong Province, China
    3Research Room of Ophthalmology, Medical College of Jinan University, Guangzhou  510630, Guangdong Province, China
  • Received:2010-12-15 Revised:2011-04-01 Online:2011-05-07 Published:2011-05-07
  • Contact: Chen Jian, Doctor, Professor, Chief physician, Department of Ophthalmology, First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China Drchenj@163.com
  • About author:Jin Ling☆, Doctor, Attending physician, People’s Hospital of Baoan District, Shenzhen 518101, Guangdong Province, China Jlingx2003@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872808*; Guangdong Key Science and Technology Planning Foundation, No. 2003A3020104*; Key Subject Foundation of Overseas Chinese Affairs Office of the State Council, No. 51205004 *

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Abstract:

BACKGROUND: Studies have found that human amniotic epithelial cells (hAECs) have a multiple differentiation capacity similar as pluripotent stem cells or embryonic stem cells. It is indicated that hAECs may be a new kinds of seed cells in tissue engineering reconstruction in the further.
OBJECTIVE: To culture the hAECs in vitro and to investigate stem cell characteristics of cultured hAECs.
METHODS: The human amniotic tissue was delivered from full term caesarean, and the highly purified hAECs were obtained from enzyme digestion method and differently sticky method. The hAECs were seeded in DMEM/F12 medium obtaining 10% fetal bovine serum for the first time and serial subcultivation. The immunofluorescence and flow cytometry were used to detect the expression of OCT-4 of stem cell factor and Stem cell surface molecule marker CD29, CD34, CD44, CD45, CD105 in passaged hAECs.
RESULTS AND CONCLUSION: The cells in vitro looked like slabstone that epithelial cells specified. The results of immunofluorescence showed the expression of OCT-4 of stem cell factor in hAECs was positive. Detection of stem cell surface molecule marker by the flow cytometry showed the expression of CD29, CD34 on hAECs were positive, and the expression of CD44, CD45, CD105 on hAECs were negative. The findings show that the hAECs have some characteristics of embryonic stem cells, and may be a new kind of seed cells for the future tissue engineering reconstitution.

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