Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3489-3493.doi: 10.3969/j.issn.1673-8225.2011.19.016

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Umbilical cord mesenehymal stem cells transplantation for the immune system following systemic lupus erythematosus

Xiao Yu-cui1, Wang Ji-bo1, Dong Jing1, Gao Hong2, Liang Hong-da1, Wang Li2, Xin Miao-miao1   

  1. 1Department of Rheumatology, Affiliated Hospital of Qingdao University Medical School, Qingdao  266003, Shandong Province, China; 2Stem Cell Research Center, Affiliated Hospital of Qingdao University Medical School, Qingdao  266003, Shandong Province, China
  • Received:2010-12-31 Revised:2011-02-19 Online:2011-05-07 Published:2011-05-07
  • Contact: Wang Ji-bo, Doctor, Chief physician, Department of Rheumatology, Affiliated Hospital of Qingdao University Medical School, Qingdao 266003, Shandong Province, China wangjibo2005@126.com Correspondence to: Dong Jing, Doctor, Associate chief physician, Department of Rheumatology, Affiliated Hospital of Qingdao University Medical School, Qingdao 266003, Shandong Province, China dongjing218@yahoo.com.cn
  • About author:Xiao Yu-cui★, Studying for master’s degree, Department of Rheumatology, Affiliated Hospital of Qingdao University Medical School, Qingdao 266003, Shandong Province, China xiaoyucui1105@163.com

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Abstract:

BACKGROUND: Recently, many studies indicate that umbilical cord mesenehymal stem cells (UC-MSCs) have therapeutic effects on systemic lupus erythematosus (SLE). There are few reports, however, regarding the immunosuppressive effect and the organs of UC-MSCs on SLE . 
OBJECTIVE: To investigate the efficacy and influence of UC-MSCs transplantation on immune systems and different organs in the treatment of SLE.
METHODS: MRL/lpr female mice were divided into 4 groups: control group, cyclophosphamide (CTX) group, CTX+UC-MSCs group, and UC-MSCs group. 24-hour proteinuria was assessed using Bradford method．Enzyme-linked immunosorbent assay was used to measure the levels of serum anti-dsDNA antibodies and interleukin-10 (IL-10), IL-17, interferon gamma (IFN-γ), PDGF. The histopathology changes of the kidney were observed using hematoxylin-eosin staining, and the UC-MSCs surface marks CD105 and CD44 in the kidney, lung and spleen were observed.
RESULTS AND CONCLUSION: ①The levels of IL-10, IL-17 and IFN-γ in the CTX+UC-MSCs group was lower than those in the control group (P < 0.05). ②At 2 weeks, the 24-hour proteinuria in the CTX+UC-MSCs and UC-MSCs groups was lower than that in the CTX and control groups (P < 0.01). ③The serum level of anti ds-DNA antibodies in the CTX+UC-MSCs group was significantly decreased as compared with that in the control group. ④After UN-MSCs transplantation, glomerulosclerosis and inflammatory cells infiltrating degree relieved. ⑤At 32 weeks, the expression of CD105 and CD44 in the kidney, lung and spleen was negative. The results indicated that MSCs transplantation can reduce lupus activity of MRL/lpr mice significantly and have marked therapeutic effect for lupus nephritis. It can also inhibit immune system by reducing the levels of inflammatory factors.

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