Abstract:

BACKGROUND: Currently, the genetic resource of hepatocarcinoma has not be collected and protected well; therefore, in order to save the proband pedigrees of Guangdong Province, a information-based management of tumor database was established in Zhujiang Hospital Affiliated to Southern Medical University.
OBJECTIVE: To investigate the collection and conservation of tumor tissue specimens, and the approaches to information-based management of tumor database.
METHODS: The tumor tissues, tumor-adjacent tissues, distal normal tissues removed from surgery and patient serum and extracted nucleic acids from tumor tissues were collected. Specimens of the tissue fragments, nucleic acids, serum, plasma, lymphocyte and stem cells, etc. were respectively conserved in liquid nitrogen or -80 ℃ refrigerator. Meanwhile, a database of information management of tumor tissue bank was established, including positions of sample placement, specimen use, laboratory results, comprehensive query, management of specimen disposal, etc.
RESULTS AND CONCLUSION: Specimens of 45 cases, including relevant specimens of 5 cases of benign tumors and 40 cases of malignant tumors, were collected. The tumor tissue data base was established and managed according to the above-mentioned methods, which functioned with good results in the past 1 year. The establishment of tumor tissue database enables the quantity control of the specimens and information-based management, as well as conservation of infrequent specimens and resource share.