Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3323-3327.doi: 10.3969/j.issn.1673-8225.2011.18.023

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Endothelial microparticles for diagnosis of acute rejection following renal transplantation

Yang Jing1, Cui Jie-wei1, Li Ding-xian2, Cao Wei-ke1, Sun Yi1   

  1. 1Department of Immunology, Medical College of Chinese People’s Armed Police Force, Tianjin  300162, China
    2Health Team, Changzhou Detachment of Jiangsu General Armed Forces, Changzhou  213003, Jiangsu Province, China
  • Received:2010-10-22 Revised:2011-01-10 Online:2011-04-30 Published:2011-04-30
  • Contact: Sun Yi, Doctor, Professor, Department of Immunology, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China drsunyi@163.com
  • About author:Yang Jing★, Master, Lecturer, Department of Immunology, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China yangjingdj@hotmail.com
  • Supported by:

    the Applied Basis and Advanced Techonlogy Program of Tianjin, No. 08JCYBJC06800*; the General Program of Medical College of Chinese People’s Armed Police Force, No. WY200912*

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Abstract:

BACKGROUND: Variation of endothelial cells is closely associated with graft rejective reaction. After transplantation, endothelial microparticles (EMPs) are shed from the plasma membrane of endothelial cells, and can reflect the change of endothelial cells directly. Accordingly, it is significant to detect plasma EMPs for monitoring acute rejection after renal transplantation.   
OBJECTIVE: To study the changes of EMPs in rats of acute rejection after renal transplantation and their relations with acute rejection.
METHODS: The syngeneic and allogeneic rat models of orthotopic renal transplantation were established. Pathological changes of the kidneys were observed at 5 days after transplantation by haematoxylin-eosin staining, and were graded according to the Bnaff criteria. The expression of intercellular adhesion molecule-1 in rat kidneys was quantitatively detected by immunohistochemistry. The level of CD144+ EMPs and intercellular adhesion molecule (ICAM)-1+/CD144+ EMPs were measured by flow cytometry. The relation between quantity and phenotype of EMPs and pathological changes of kidneys was analyzed.
RESULTS AND CONCLUSION: Compared with syngeneic group, the Bnaffs grades (P < 0.01), the expression of ICAM-1 of kidney (P < 0.01) in allogeneic group was significantly increased. Compared with syngeneic group, the level of CD144+ EMPs and ICAM-1+/CD144+EMPs (P < 0.01) in allogeneic group were significantly increased. The level of CD144+ EMPs was positively related with the Bnaffs grades (P < 0.01), and the level of ICAM-1+/ CD144+EMPs was positively related with the expression of ICAM-1 of kidney (P < 0.01). The level of EMPs detection may be helpful to the diagnosis of acute rejection after transplantation.

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