Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3293-3297.doi: 10.3969/j.issn.1673-8225.2011.18.016

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Effect of bone marrow derived mesenchymal stem cells transplantation combined with cryopreservation on trachea allograft epithelium vascular endothelial growth factor levels

Han Yun1, Ji Zi-zhao1, Lan Ni2, Pang Chao-jian2, Tong Xiao-jie2, Shi Wen-jun1   

  1. 1Department of Thoracic Surgery, China Medical University Affiliated Shengjing Hospital, Shenyang  110004, Liaoning Province, China
    2Department of Anatomy, College of Basic Medicine, China Medical University, Shenyang  110001, Liaoning Province, China
  • Received:2010-10-26 Revised:2010-12-03 Online:2011-04-30 Published:2011-04-30
  • Contact: Han Yun☆, M.D, Ph.D., Attending surgeon, Department of Thoracic Surgery, China Medical University Affiliated Shengjing Hospital, Shenyang 110004, Liaoning Province, China ryan.hanyun@gmail.com
  • About author:Han Yun☆, M.D, Ph.D., Attending surgeon, Department of Thoracic Surgery, China Medical University Affiliated Shengjing Hospital, Shenyang 110004, Liaoning Province, China ryan.hanyun@gmail.com
  • Supported by:

    Science and Technology Research Program of Liaoning Educational Committee, No. L2010606*

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Abstract:

BACKGROUND: Cryopreserved tissues can maintain their vitality and integrity, and reduce the antigenicity.
OBJECTIVE: Using intravenous transplantation of bone marrow derived mesenchymal stem cells (BMSCs) combined with cryopreserved allograft, to investigate the role of BMSCs on enhancing the implant survival and revascularization by measuring vascular endothelial growth factor (VEGF) levels.
METHODS: After transplanted with cryopreserved 2 weeks and 6 weeks allocraft, PKH-26 labeled 3-5 passage BMSCs were injected into the recipient rats via the tail vein. Rats in the control groups were injected with the same amount of phosphate buffer solution (PBS). We observed the histology of the transplanted trachea and measured the VEGF protein levels in the epithelium to evaluate the role of BMSCs transplantation on the epithelium revascularization.
RESULTS AND CONCLUSION: Rats from BMSCs injection group survived a long period. Histological observation showed that the tracheal lumen was covered by psudo-striated ciliated columnar epithelium. VEGF protein levels in the BMSCs injection group were higher than PBS group. BMSCs may exert their reparation function via enhancing epithelium VEGF expression and revascularization.

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