Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3239-3242.doi: 10.3969/j.issn.1673-8225.2011.18.003

Previous Articles     Next Articles

Pre-infusion of donor apoptotic lymphocytes effects on receptor CD4+T, CD8+T, CD4+CD25+ T regulatory cells during porcine liver transplantation

Zhang Qing-jun1, Sun Ke-jian1, Chen Yun1, Xie Jin-min2, Gao Yi2   

  1. 1Department of Hepatobiliary Surgery, Central Hospital of Zibo City, Zibo  255005, Shandong Province, China
    2Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2010-11-29 Revised:2011-04-06 Online:2011-04-30 Published:2011-04-30
  • Contact: Gao Yi, Doctor, Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China zhang72128@163. com
  • About author:Zhang Qing-jun☆, Studying for doctorate, Department of Hepatobiliary Surgery, Central Hospital of Zibo City, Zibo 255005, Shandong Province, China zhang72128@163. com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. C03020504*

PDF

407

Abstract:

BACKGROUND: Apoptotic cells can actively regulate the body’s immune function, and induce immune tolerance through regulation of cellular and humoral immune, which has been only confirmed in rat liver transplantation models.
OBJECTIVE: To investigate the influence of CD4+T, CD8+T, CD4+CD25+ T regulatory cells of preoperative transfusion of lymphocyte from in vitro 60Coγ-treated donor porcine on allograft survival of orthotopic liver transplantation (OLT).
METHODS: OLT models were performed without veno-venous bypass (VVB) in Chinese inbred miniature porcine. The recipient porcine were administrated intravenously with donor apoptotic lymphocyte (5×108) 7 days before liver transplantation, or not treated. Changes of CD4+T, CD8+T, CD4+CD25+ T regulatory cells and liver pathological changes were observed after the transplantation. Apoptotic percentage of lymphocytes from in vitro 60Coγ-treated was measured by flow cytometry using AnnexinⅤ/PI double staining method.
RESULTS AND CONCLUSION: Liver grafts biopsy of two group showed the same change after operation, there were mild or serious acute rejections on the 3rd day after operation, and serious acute rejection on the 6th day. The increasing changes of CD4+T, CD8+T and CD4+CD25+T regulatory cells were just like the change of lymphocytes. But the contrast of two group was of non-significance (P >0.05). These indicate that immunological tolerance cannot be induced by transfusion of preoperative donor 60Coγ-treated lymphocytes in porcine liver allograft, preoperative transfusion of lymphocytes cannot influence the expression of CD4+T, CD8+T, CD4+CD25+ T regulatory cells in tolerance receptor of porcine liver transplantation.

CLC Number: