Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3231-3234.doi: 10.3969/j.issn.1673-8225.2011.18.001

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Monitoring of liver cell metabolism in early transplantation with the multi parameter system biosensor Neurotrend-7

Tang Ji-hong1, Tang Bo1, Zhang Jie1, Yang Da-kuan1, Tian Da-guang1, Fu Bi-mang1, Li Han-yu2, Yu Sheng-feng1, Zhu Hong1   

  1. 1Department of Hepatobiliary-pancreatic Surgery, Second Affiliated Hospital of Kunming Medical College, the Institute of Hepatobiliary- Pancreatic Surgery, Second Affiliated Hospital of Kunming Medical College, Kunming  650101, Yunnan Province, China
    2the Second People’s Hospital of Yunnan Province, Kunming  650101, Yunnan Province, China
  • Received:2010-11-01 Revised:2010-12-21 Online:2011-04-30 Published:2011-04-30
  • Contact: Zhu Hong, Doctor, Associate professor, Department of Hepatobiliary-pancreatic Surgery, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, Yunnan Province, China Correspondence to: Zhang Jie, Master, Professor, Doctoral supervisor, Department of Hepatobiliary-pancreatic Surgery, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, Yunnan Province, China zhangjie@public.km.yn.cn
  • About author:Tang Ji-hong, Department of Hepatobiliary-pancreatic Surgery, Second Affiliated Hospital of Kunming Medical College, the Institute of Hepatobiliary- pancreatic Surgery, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, Yunnan Province, China tangbo1227@163.com
  • Supported by:

    Fund of Kunming Medical College United Yunnan Provincial Technology Commission, No. 2007C0011R*

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Abstract:

BACKGROUND: At present, various monitoring equipments have been used in graft early monitoring, but there is no way to continuous monitoring transplant liver cell metabolic changes to reflect the graft internal environment changes.
OBJECTIVE: To explore continuous monitoring of liver cell metabolism with multi parameter system biosensor (Neurotrend-7), and to provide the basis for the liver hypofunction after transplantation in early stage.
METHODS: A total of 24 healthy, adult, male, New Zealand, White rabbits were divided into 3 groups: control group, experimental group 1, and experimental group 2. Control group (n=4): They were treated with buried oxygen metabolism monitoring sensor of liver tissue when abdomen was opened, and abdomen was closed, after the specimen drawn. Multi parameter system biosensor probe was inserted into liver after liver transplantation between experimental group 1 and experimental group 2. Ten cases of rabbits underwent the liver transplantation, for the liver retention time was lesser than 2 hours in experimental group 1; 4 cases of rabbits underwent the liver transplantation, for the liver retention time was more than 4 hours in experimental group 2. Before transplantation and 1, 3, 5 days after transplantation, the changes of the pH, PCO2, and PO2 value in liver tissue were continually monitored, and PaO2, PaCO2, and arterial blood gas (ABG) pH value were measured. At the same time, the liver tissue cut in the corresponding points was observed under the optical microscope and electron microscope.
RESULTS AND CONCLUSION: PO2 value of liver tissue in experimental group 2 was significantly lower than that in experimental group 1 (P < 0.05), and gradually decreased with the time. However, PCO2 value of liver tissue in experimental group 2 at each time was significantly higher than that in experimental group 1 (P < 0.05), and gradually increased with the time. pH value of liver tissue in experimental group 2 at each time was lower than that in experimental group 1 (P < 0.05). It is indicated that liver function is serious adverse and transplant is non-function. The PO2 and PaO2 were positive correlation; however, PCO2 and PaCO2 were not obvious relevance. Also, the pH value and ABG pH value were not obvious relevance. Changes are similar to the histology inspection. It suggested that multi parameter system biosensor (Neurotrend-7) can discover liver hypofunction after transplantation at early stage and observes its evolution rule, to guide early prevention and the treatment in clinic, it also can provides the accurate data for clinical application in the liver surgery and organ transplantation field.

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