Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2821-2824.doi: 10.3969/j.issn.1673-8225.2011.15.039

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Strategies for fragment antigen binding expression in Escherichia coli

Liu Yun-hong, Hou Zong-liu   

  1. Central Laboratory, Department of Scientific Research, Yan’an Affiliated Hospital of Kunming Medical University, Kunming   650051, Yunnan Province, China
  • Received:2010-10-07 Revised:2011-02-12 Online:2011-04-09 Published:2013-11-06
  • Contact: Hou Zong-liu, Professor, Central Laboratory, Department of Scientific Research, Yan’an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan Province, China hzl579@163. com
  • About author:Liu Yun-hong★, Studying for master’s degree, Laboratorian, Central Laboratory, Department of Scientific Research, Yan’an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan Province, China liuyunhong2001@ 163.com
  • Supported by:

    Graduate Innovation Fund of Kunming Medical University. No. 2009J10*

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Abstract:

BACKGROUND: Low-yields of antigen-binding fragment (Fab) restricts its full scale operation and application, which may be changed by remodeling or optimizing host cells, expression vectors, expression condition and purification conditions. 
OBJECTIVE: To summarize and introduce new strategies about expression and purification of Fab antibodies in Escherichia coli (E. coli).
METHODS: CNKI and Medline databases were searched by the first author using key words of “Escherichia coli, Fab, Expression, antibody, purification” in Chinese or English from year 1990 to 2010. The Fab antibody expression and purification in E. coli were summed up and new researches concerning Fab antibody expression and purification in E. coli were introduced.
RESULTS AND CONCLUSION: Totally 125 papers were found and 30 papers were included. The results show that the Fab antibodies are mainly by cytoplasmic expression and secreted expression in E. coli. Its purification methods are mainly by metal ions and chelating affinity purification and specific proteins affinity purification. The influence factors concerning Fab expression in E. coli are correlated to each other. A substantial increase in current research on the latest Fab antibodies in E. coli expression and purification strategies are not consistent optimal, there is no best, only the most suitable.

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