Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2781-2785.doi: 10.3969/j.issn.1673-8225.2011.15.029

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Effect of herbal ultrasound-phoresis on bone morphogenetic protein 7 expression during radialis fracture healing

Qiu Yu, Gao Shi-chang, Ni Wei-dong, Pu Chao   

  1. Department of Orthopaedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Received:2010-11-16 Revised:2011-03-04 Online:2011-04-09 Published:2013-11-06
  • Contact: Gao Shi-chang, Doctor, Associate professor, Associate chief physician, Master’s supervisor, Department of Orthopaedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China gaoshichang2002@yahoo.com.cn
  • About author:Qiu Yu★, Studying for master’s degree, Department of Orthopaedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China 47022189@qq.com
  • Supported by:

    Chongqing Health Bureau for Traditional Chinese Medicine, No. 2008-2-39*

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Abstract:

BACKGROUND: Some scholars combined low-intensity pulsed ultrasound (LIPUS) with herbal (herbal ultrasound-phoresis) and this therapy had a good curative effect while being used for treating fractures in animal experiment and clinical treatment. However, there is fewer report about the mechanism that herbal ultrasound-phoresis promotes fracture healing.
OBJECTIVE: To investigate the effect on bone morphogenetic protein 7 (BMP-7) expression during fracture healing treated by herbal ultrasound-phoresis in rabbits.
METHODS: Animal models of radius fracture in rabbits were established. Totally 84 rabbits of New Zealand were divided into 3 groups after successful model preparation. The experimental group were treated by herbal ultrasound-phoresis, the ultrasound group were treated by LIPUS, the control group were given sham exposure. The rabbits were sacrificed at 3, 6, 9, 12, 15 days and 4, 6 weeks respectively after operation. The changes of BMP-7 mRNA expression and mean absorbance value were detected by RT-PCR and immunohistochemical examinations; the fracture healing was assessed through X-ray at 4 and 6 weeks after operation.
RESULTS AND CONCLUSION: X-ray showed that the fracture in the experimental group was healed faster than the ultrasound group and the control group. The expressions of BMP-7 mRNA and mean absorbance value in the experimental group were higher than that in ultrasound group and the control group (P < 0.05), which was particularly obvious at 9 ,12 days (P < 0.01). Herbal ultrasound-phoresis might accelerate fracture healing by increasing the expression of BMP-7.

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