Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2672-2676.doi: 10.3969/j.issn.1673-8225.2011.15.004

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Interventional effect of calcitonin on insulin like growth factor 1 gene expression in osteoblasts under simulated microgravity

Su Jia-lin1, Zhang Hua1, Yang Li-ting2, Yang Rui1, Wang Mai-tao1, Huang Zhen3   

  1. 1Department of Endocrinology, 2Department of Hematology, 3Department of Surgery, Zhujiang Hospital, Southern Medical University Guangzhou  510282, Guangdong Province, China
  • Received:2010-09-16 Revised:2010-12-01 Online:2011-04-09 Published:2013-11-06
  • Contact: Huang Zhen, Professor, Master’s supervisor, Department of Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China hzhen2@sina.com
  • About author:Su Jia-lin★, Studying for master’s degree, Physician, Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China karim173@yahoo.com.cn

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Abstract:

BACKGROUND: Space flight, bed-ridden state of low-gravity load will cause bone metabolic disorder which includes bone absorption and bone formation abnormal, leading to bone loss, decreased bone mineral density. Researches have showed that calcitonin can promote osteoblastic activity and mRNA expression of insulin like growth factor 1 (IGF-1).
OBJECTIVE: To observe the effects of calcitonin on osteoblastic activity and mRNA expression of IGF-1 in vitro under simulated microgravity.
METHODS: Osteoblasts were collected from newborn SD mouse by means of double enzyme digestion. The osteoblasts were polymerized and encapsulated the cells inside alginate carriers in Cacl2 solution. All cells were assigned into normal gravity, simulated microgravity and simulated microgravity + calcitonin (10, 40 and 80 IU/L) groups. Osteoblasts were cultured for 72 hours in simulated microgravity and normal gravity in the three-dimensional rotating vessel. The proliferation and apoptosis of the osteoblasts and alkaline phosphatase (ALP) activity were detected, in addition, the expression of IGF-1, ALP and osteocalcin were measured by RT-PCR.
RESULTS AND CONCLUSION: Compared with the normal gravity group, the osteoblasts proliferation rate and secretion of ALP were decreased, and the expression of IGF-1, ALP, osteocalcin mRNA were reduced in the simulated microgravity group (P < 0.01). Compared with the simulated microgravity group, osteoblasts proliferation rate, IGF-1, ALP, osteocalcin mRNA were increased in the simulated microgravity + calcitonin group, but no dose-dependent manner could be found. The findings demonstrated that calcitonin can improve osteoblast proliferation and secretion functions via up-regulating IGF-1 mRNA expression in simulated microgravity.

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