Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 187-190.doi: 10.3969/j.issn.1673-8225.2011.01.043

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Surface marker changes in human umbilical cord-derived mesenchymal stem cells after cryopreservation and resuscitation

Qian Yan-xiang1, Shu Qun2, Cai Hong-xia2, Guo Juan3, Chen Liang1, Gao Feng1   

  1. 1Shanghai Cord Blood Bank, Shanghai  200051, China
    2Changning District Maternal and Child Health Hospital, Shanghai   200051, China
    3Department of Hematology, Shanghai Sixth People’s Hospital, Shanghai   200233, China
  • Received:2010-07-06 Revised:2010-11-01 Online:2011-01-01 Published:2011-01-01
  • About author:Qian Yan-xiang★, Master, Shanghai Cord Blood Bank, Shanghai 200051, China qianyx1982@gmail.com

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Abstract:

BACKGROUND: Mesenchymal stem cells are the stem cells that possess the capability for self-renewal and multi-directional differentiation. Umbilical cord is the tissue outside the embryos and would be fallen off after parturition. In addition, it has wide source and no ethical restriction, so it is promising to be the first choice for mesenchymal stem cells. 
OBJECTIVE: To detect the surface markers CD29, CD44, CD49e, CD73, CD90, CD34, CD45, and CD271 of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) prior to and after cryopreservation and resuscitation.
METHODS: After isolation and culture, morphology of the primary, P4 and P8 hUCMSCs was observed prior to cryopreservation and after resuscitation. Surface markers CD29, CD44, CD49e, CD73, CD90, CD34, CD45, and CD271 of primary, P4, and P8 hUCMSCs were detected through the use of flow cytometry prior to cryopreservation and after resuscitation
RESULTS AND CONCLUSION: hUCMSCs prior to cryopreservation and hUCMSCs of different passages after resuscitation present the same phenotype, i.e., positive for CD29, CD44, CD49e, CD73, and CD90, and negative for CD34, CD45, CD271. These findings suggest that primary hUCMSCs do not present changes in surface markers after cryopreservation and resuscitation.

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