Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (26): 5520-5527.doi: 10.12307/2025.768

Previous Articles     Next Articles

Protective mechanism of nitrooleic acid on submandibular gland cell radiation injury in rats

Lin Peiqi1, 2, Luo Qinliang3, Zhang Ligang1, Huang Guilin3, Tang Jianhong1, Zhang Nini1   

  1. 1Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China; 2Department of Stomatology, Longyan First Hospital, Longyan 364000, Fujian Province, China; 3Department of Stomatology, Fifth Affiliated Zhuhai Hospital of Zunyi Medical University, Zhuhai 519110, Guangdong Province, China
  • Received:2024-08-14 Accepted:2024-10-22 Online:2025-09-18 Published:2025-02-20
  • Contact: Zhang Nini, MS, Associate professor, Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • About author:Lin Peiqi, MS, Physician, Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China; Department of Stomatology, Longyan First Hospital, Longyan 364000, Fujian Province, China
  • Supported by:
     National Natural Science Foundation of China, No. 81960204 (to HGL); Guizhou Provincial Key Clinical Construction Project, No. [2017]24 (to HGL); Guizhou Provincial Science and Technology Plan Project, No. ZK[2024]339 (to ZNN); Zunyi Medical University “Future Clinical Famous Doctor” Project, No. 20211017 (to ZNN); Guizhou Provincial Education Department Youth Science and Technology Talent Growth Project, No. KY[2022]291 (to ZLG); Guizhou Provincial Health Commission Science and Technology Fund Project, No. gzwjkj2020-1-165 (to ZLG)

PDF

91

Abstract: BACKGROUND: Recent studies have found that Nrf2/ARE signaling pathway activators have the characteristics of low toxicity and control, and have a protective effect against radiation tissue damage.
OBJECTIVE: To investigate whether nitrooleic acid can protect submandibular gland epithelial cells from radiation injury by regulating the Nrf2/ARE signaling pathway.
METHODS: Rat submandibular gland epithelial cells were cultured in vitro and CCK-8 assay was used to screen the optimal concentration and time of nitrooleic acid administration. Submandibular gland epithelial cells were divided into non-radiation group, radiation control group, nitrooleic acid group, nitrooleic acid + ML385 (Nrf2/ARE signaling pathway specific inhibitor) group, and ML385 group. Submandibular gland cells were pretreated with nitrooleic acid and ML385 for 24 hous according to the experimental groups, and then irradiated with 5 Gy radiation to establish the models. At 48 hours after irradiation, CCK-8 assay was used to detect the cell proliferation rate. Real-time quantitative PCR was used to detect the expression of Nrf2, HO-1, and NQO1 mRNA in the cells. Real-time quantitative PCR and enzyme-linked immunosorbent assay were used to detect the cell secretion function and the expression of inflammatory factors. DCFH-DA fluorescent probe kit was used to detect the level of intracellular reactive oxygen species. 
RESULTS AND CONCLUSION: (1) Compared with the radiation control group, the proliferation rate of submandibular gland epithelial cells and the expression levels of secretion function related factors aquaporin 5 and α-amylase in the nitrooleic acid group of rats increased (P < 0.05), and the expression levels of Nrf2, HO-1, and NQO1 mRNA increased (P < 0.05), while the expression levels of inflammatory factors interleukin-1β, interleukin-6, and tumor necrosis factor-α decreased (P < 0.05), and reactive oxygen species generation reduced (P < 0.01). (2) Compared with the nitrooleic acid group, the addition of nitrooleic acid and ML385 group resulted in a decrease in cell proliferation rate and expression levels of secretion function related factors aquaporin 5 and α-amylase (P < 0.05), and mRNA expressions of Nrf2, HO-1, and NQO1 were all decreased (P < 0.05), while the expression levels of inflammatory factors interleukin-1β, interleukin-6, and tumor necrosis factor-α increased (P < 0.05), and generation of reactive oxygen species increased (P < 0.05). (3) Results indicated that in the radiation environment, nitrooleic acid has a certain protective effect on the proliferation ability and secretion function of rat submandibular gland epithelial cells, reduces the expression of inflammatory factors, lowers intracellular reactive oxygen species levels, and alleviates the damage of rat submandibular gland epithelial cells caused by radiation. This function may be related to the activation of Nrf2/ARE signaling pathway. 

Key words: submandibular gland epithelial cell, radiation damage, nitrooleic acid, Nrf2/ARE signaling pathway, ionizing radiation, radiation damage, antioxidative stress, engineered cell

CLC Number: