Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (13): 1983-1988.doi: 10.12307/2024.161

Previous Articles     Next Articles

Effects of micro-electric field on proliferation and osteogenic differentiation of human umbilical cord mesenchymal stem cells

Liu Zhong1, Li Kewei1, Wang Min2, Liu Wenhui2, Zhang Leilei2, Guo Song1, Qian Hui2, Fu Qiang1   

  1. 1Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China; 2School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China
  • Received:2023-03-27 Accepted:2023-05-22 Online:2024-05-08 Published:2023-08-28
  • Contact: Fu Qiang, Chief physician, Associate professor, Doctoral supervisor, Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China Qian Hui, Professor, Doctoral supervisor, School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China
  • About author:Liu Zhong, Master candidate, Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China Li Kewei, Master candidate, Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China
  • Supported by:
    National Natural Science Foundation of China, No. 81971154 (to FQ)

PDF

99

Abstract: BACKGROUND: Electrical stimulation is a physical method that can be used to induce various cellular activities such as cell proliferation, differentiation, and apoptosis. The induction of osteogenic differentiation of stem cells will be beneficial in the field of bone regeneration.
OBJECTIVE: To observe whether micro-current field can promote the proliferation and osteogenic differentiation of human umbilical cord mesenchymal stem cells.
METHODS: The fresh human umbilical cord tissue was cut to obtain umbilical cord mesenchymal stem cells, which were inoculated into a 6-well plate after cell culture and passage to the third generation. After 24 hours, the cells were cultured under a stimulation of 0, 50, and 100 mV/mm micro-electric field, at a frequency of 1 hour per day for 3 continuous days. The growth and morphological changes of human umbilical cord mesenchymal stem cells were observed by a microscope. The cell proliferation was detected by CCK-8 assay and EdU staining. Alizarin red staining was used to detect the osteogenic differentiation ability of cells. Western blot assay was used to determine the expression of ERK signal pathway proteins. 
RESULTS AND CONCLUSION: (1) The optical density value and the number of proliferating cells in 50 and 100 mV/mm groups were significantly higher than those of the unstimulated group (P < 0.05). (2) Human umbilical cord mesenchymal stem cells could be induced to differentiate into osteocytes before and after micro-electric field stimulation, but the differentiation rate of 50 and 100 mV/mm groups was faster than that of unstimulated groups. (3) The protein expression of p-ERK1/2 in the 50 and 100 mV/mm groups was higher than that in the unstimulated group, and significant difference was detected between the 100 mV/mm group and the unstimulated group (P < 0.05). (4) Micro-electric field can promote the proliferation of human umbilical cord mesenchymal stem cells, and the mechanism may be achieved by promoting the phosphorylation of ERK.

Key words: micro-electric field, cell proliferation, human umbilical cord mesenchymal stem cell, cell differentiation

CLC Number: