Effect of osteoclast TRPV5 channel on in vitro degradation of biological coral artificial bone

doi:10.12307/2023.413

Abstract

Abstract: BACKGROUND: Biological coral artificial bone is a good alternative material for repairing long bone defects. However, the degradation and absorption rate of biological coral artificial bone in the human body does not match the growth rate of new bone, which leads to the limitation of its clinical application.
OBJECTIVE: To explore the effect of osteoclast TRPV5 on the degradation of biological coral artificial bone.
METHODS: Monocyte macrophage strain RAW264.7 from mouse was inoculated on biological coral artificial bone slices, which were co-cultured with osteoclasts. After the appearance of osteoclasts was observed, the osteoclast-induced differentiation medium containing 0, 50, 500, 5 000 µmol/L ruthenium red (TRPV5 channel inhibitor) was added separately. After culturing for a certain period of time, the expression of TRPV5 channel protein was observed under a laser confocal microscope. The absorption lacuna on the biological coral artificial bone slice was observed under a scanning electron microscope. The expression of TRPV5 channel protein in cells was detected by western blot assay.
RESULTS AND CONCLUSION: (1) Laser scanning confocal microscope: TRPV5 could be expressed on the cytoplasm and membrane of osteoclasts. With the increase of ruthenium red concentration, the expression of TRPV5 on osteoclasts decreased. (2) Scanning electron microscope: Large continuous bone resorption lacuna was seen on the bone slices in the 0 nmol/L ruthenium red group. The bone resorption lacuna on the bone slices in other concentrations of ruthenium red decreased and was scattered, and with the increase of the concentration of ruthenium red, the area of the bone lacuna gradually decreased. (3) Western blot assay: Compared with 0 nmol/L ruthenium red group, the expression of TRPV5 channel protein in other concentrations of ruthenium red groups was significantly decreased (P < 0.05 or P < 0.01). (4) The results confirm that osteoclasts grow well on biological coral artificial bone, and TRPV5 can be used as a target to regulate the degradation rate of biological coral artificial bone.

Key words: biological coral artificial bone, degradation, osteoclast, TRPV5 channel, bone defect

CLC Number:

Cui Hongwang, Wang Liangsheng, Wen Peng, Meng Zhibin. Effect of osteoclast TRPV5 channel on in vitro degradation of biological coral artificial bone[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(21): 3337-3342.

Figures/Tables 4

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