中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (49): 9209-9213.doi: 10.3969/j.issn.2095-4344.2012.49.016

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓间充质干细胞修复Wistar大鼠的脊髓损伤

贾全章1，李东君2，陈玉丙3，孙景海1，王风华1，徐 爽1，刘丽萍3，高德萱2，姜大伟2   

  1. 1解放军第208医院，吉林省长春市 130062；2延边大学医学部，吉林省延吉市 133002；3吉林大学附属第二医院，吉林省长春市 130041
  • 收稿日期:2012-05-04 修回日期:2012-06-22 出版日期:2012-12-02 发布日期:2012-06-22
  • 作者简介:贾全章☆，男，1964年生，山东省枣庄市人，汉族，2005年解放军第三军医大学毕业，博士，主任医师，主要从事脊柱外科、脊髓损伤的研究。jqz208@sohu.com
  • 基金资助:

    吉林省科技发展计划项目(20080446)。

Bone marrow mesenchymal stem cells repair Wistar rat spinal cord injury  

Jia Quan-zhang1, Li Dong-jun2, Chen Yu-bing3, Sun Jing-hai1, Wang Feng-hua1, Xu Shuang1, Liu Li-ping3,
Gao De-xuan2, Jiang Da-wei2   

  1. 1No.208 Hospital of Chiinese Liberation Army, Changchun 130062, Jilin Province, China; 2Department of Medicine, Yanbian University, Yanji 133002, Jilin Province, China; 3The Second Affiliated Hospital of Jilin University, Changchun 130062, Jilin Province, China
  • Received:2012-05-04 Revised:2012-06-22 Online:2012-12-02 Published:2012-06-22
  • About author:Jia Quan-zhang☆, Doctor, Chief physician, No.208 Hospital of Chinese Liberation Army, Changchun 130062, Jilin Province, China jqz208@sohu.com
  • Supported by:

    jlin Science and Technology Development Planning Program, No. 20080446*

PDF

553

被引次数

4

摘要：

背景：研究表明，骨髓间充质干细胞移植治疗脊髓损伤已取得疗效。
目的：观察骨髓间充质干细胞移植对脊髓损伤大鼠脊髓功能的影响。
方法：120只大鼠随机数字表法分为4组：空白组不造模，于脊髓损伤后当天损伤区局部注射生理盐水10 μL；模型组采用改良的Allen’s打击法造成脊髓损伤模型，不做任何治疗；低浓度治疗组行脊髓损伤后当天经尾静脉注射浓度为1×109 L-1骨髓间充质干细胞1 mL；高浓度治疗组行脊髓损伤后当天损伤区局部注射浓度为4×1010 L-1骨髓间充质干细胞10 μL。
结果与结论：损伤后1 d模型组和两治疗组均无恢复迹象，运动评分低于空白组(P < 0.01)；损伤7 d，两治疗组运动功能有所恢复，行为学评分均高于模型组，但差异无显著性意义(P > 0.05)；损伤15，30 d，两治疗组行为学评分均明显高于模型组(P < 0.01)。移植后7，15，30 d脊髓病理切片显示治疗组较模型组均有显著恢复。提示骨髓间充质干细胞对脊髓损伤模型大鼠的脊髓功能有修复作用。

关键词: 骨髓间充质干细胞, 修复, 脊髓损伤, 细胞移植, 大鼠, 静脉移植, 局部注射, 细胞浓度, 干细胞, 运动功能

Abstract:

BACKGROUND: Studies have shown that bone marrow mesenchymal stem cells transplantation has achieved therapeutic effects in the treatment of spinal cord injury.
OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cells transplantation on motor function of rats with spinal cord injury.
METHODS: 120 Wistar rats were randomly divided into four groups: (1) in the blank group, rats were not modeled but they were locally injected with 10 μL normal saline at the day of spinal cord injury; (2) in the model group, spinal cord injury models were established by modified Allen’s crack method without any treatment; (3) in the low dose treatment group, 1 mL bone marrow mesenchymal stem cells (1×109/L) were injected through the tail vein at the day of spinal cord injury; in the high dose treatment group, rats were locally injected with 10 μL bone marrow mesenchymal stem cells (4×109/L) at the day of spinal cord injury.
RESULTS AND CONCLUSION: At 1 day after injury, there was no healing in the model group and two treatment groups, and the scores of motion function in the three groups were lower than those in the blank group (P < 0.01); at 7 days after injury, the scores of motion function in the two treatment groups were increased, and the behavior scores of the two treatment groups were higher than those of the model group, but there was no significant difference (P > 0.05); at 15 and 30 days after injury, the behavior scores of the two treatment groups were higher than those of the model group (P < 0.01). At 7, 15 and 30 days after transplantation, there was significant recovery in behaviors in the two treatment groups compared with the model group. It indicates that bone marrow mesenchymal stem cells have therapeutic effects on the spinal function of rats with spinal cord injury. 

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