中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (21): 3346-3351.doi: 10.3969/j.issn.2095-4344.2017.21.011

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓间充质干细胞移植联合生化止血方治疗产后子宫复旧不全

陈继龙，冯 筠   

  1. 广州中医药大学深圳临床医学院，广东省深圳市 518000
  • 修回日期:2017-06-11 出版日期:2017-07-28 发布日期:2017-08-02
  • 通讯作者: 冯筠，教授，主任医师，广州中医药大学深圳临床医学院，广东省深圳市 518000
  • 作者简介:陈继龙，男，1988年生，湖南省浏阳市人，汉族，在读硕士，主要从事妇产科方面的研究。
  • 基金资助:

    深圳市科技计划项目(卫生医疗类) (20130213)

Bone marrow mesenchymal stem cell transplantation combined with Shenghuazhixue decoction in the treatment of postpartum subinvolution of uterus

Chen Ji-long, Feng Jun   

  1. Shenzhen Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen 518000, Guangdong Province, China
  • Revised:2017-06-11 Online:2017-07-28 Published:2017-08-02
  • Contact: Feng Jun, Professor, Chief physician, Shenzhen Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen 518000, Guangdong Province, China
  • About author:Chen Ji-long, Studying for master’s degree, Shenzhen Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen 518000, Guangdong Province, China
  • Supported by:

    the Science and Technology Program of Shenzhen, No. 20130213

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被引次数

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摘要：

文章快速阅读：

文题释义：
产后子宫复旧不全：分娩后依靠子宫体肌纤维收缩及缩复作用，使子宫局部血液供应显著减少，子宫肌细胞缺血发生自溶而逐渐缩小，胞质减少，子宫体积亦明显缩小，子宫腔内的胎盘剥离面随着子宫的逐渐缩小而相应缩小，加之子宫内膜的再生使剥离面得以修复，子宫通常在产后五至六周时恢复到接近非孕时状态，这个过程称为子宫复旧。当产后子宫处于病理性状态的时候，这种复旧功能将受到阻碍，即发生子宫复旧不全。
骨髓间充质干细胞移植联合生化止血方促进产后子宫复旧的机制：抑制产后子宫内膜的炎症反应，促进子宫内膜修复，减轻产后子宫出血状态，抑制出血的同时，促进子宫内膜新生血管形成，促进子宫修复。

 

摘要
背景：经查阅大量文献发现，骨髓间充质干细胞移植能促进损伤子宫的修复，生化止血方具有活血化瘀，调经止痛的功效。
目的：探讨骨髓间充质干细胞移植联合生化止血方治疗子宫复旧不全的作用及其机制。 
方法：40只雌性SD大鼠建立子宫复旧不全模型，随机分为4组(n=10)：对照组、生化止血方组、骨髓间充质干细胞组、骨髓间充质干细胞+生化止血方组(联合组)。对照组不作任何处理，骨髓间充质干细胞组与联合组在造模成功后的第1天，将细胞浓度为1×107 L-1的骨髓间充质干细胞溶液1 mL局部注射至子宫肌层内；生化止血方组切开大鼠子宫后不做任何处理，生理盐水冲洗后关腹；生化止血方组与联合组分别在造模成功后的第1天开始灌喂生化止血方，灌喂体积为8 mL/(kg•d)，每日灌胃1次，连续7 d，进行相关指标检测。
结果与结论：①治疗后，联合组雌鼠体质量、子宫湿质量、子宫指数均下降，与对照组比较，差异有显著性意义(P < 0.05)，生化止血方组雌鼠体质量、子宫湿质量下降，与对照组比较，差异有显著性意义(P < 0.05)；②治疗后，生化止血方组白细胞介素1表达水平、血清肿瘤坏死因子α水平均下降，血清血栓素B2水平上升，与对照组比较，差异有显著性意义(P < 0.05)；骨髓间充质干细胞移植组白细胞介素1表达水平、血清肿瘤坏死因子α水平均下降，血管内皮生长因子水平上升，与对照组比较，差异有显著性意义(P < 0.05)，联合组白细胞介素1表达水平，血清肿瘤坏死因子α水平下降，血清血栓素B2、血管内皮生长因子水平上升，与对照组比较，差异有非常显著性意义(P < 0.01)；③结果表明，骨髓间充质干细胞移植联合生化止血方能够降低产后雌鼠体质量、子宫湿质量和子宫指数，促进子宫复旧，这种机制可能是通过降低白细胞介素1表达水平、血清肿瘤坏死因子α水平，同时升高血栓素B2、血管内皮生长因子水平实现的。

关键词: 干细胞, 移植, 子宫复旧不全, 骨髓间充质干细胞, 细胞移植, 生化止血方

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cell (BMSCs) transplantation has been reported to promote the repair of uterine injury; Shenghuazhixue decoction functions to promote blood circulation and remove blood stasis, and dysmenorrhea.

OBJECTIVE: To investigate the effect of BMSCs transplantation combined with Shenghuazhixue decoction on subinvolution of uterus effect and the underlying mechanism.
METHODS: The model of subinvolution of uterus was established in 40 female Sprague-Dawley rats, followed by randomly divided into four groups: control, Shenghuazhixue decoction, BMSCs, and combination groups. The control group received no treatment; at the 1st day after modeling, 1×107/L BMSCs (1 mL) were injected into the rat myometrium in the BMSCs and combination groups before laparotomy; the rats in the Shenghuazhixue decoction group received no treatment after incision of uterus, and then rinsed using normal saline before closing the abdomen; the Shenghuazhixue decoction and combination groups were given 8 mL/(kg•d) Shenghuazhixue detection via gavage at the 1st day after modeling, once daily, for consecutive 7 days. Afterwards, the relevant indexes were detected. RESULTS AND CONCLUSION: Compared with the control group, the body weight, uterine wet weight and uterine index in the combined group were significantly decreased after treatment (P < 0.05). The body weight and uterine wet weight showed significant differences between Shenghuazhixue decoction and control groups (P < 0.05). Compared with the control group, the expression levels of intrerleukin-1 and tumor necrosis factor-α were significantly decreased, and the serum level of thromboxane B2 was significantly increased in the Shenghuazhixue decoction group after treatment (P < 0.05). After treatment, the expression levels of intrerleukin-1 and tumor necrosis factor-α were significantly decreased, and the level of vascular endothelial growth factor was significantly increased in the BMSCs group compared with the control group (P < 0.05). Compared with the control group, there was significant decease in the expression levels of intrerleukin-1 and tumor necrosis factor-α, and significant increase in serum level of thromboxane B2 and vascular endothelial growth factor in the combination group (P < 0.01). These results suggest that BMSCs transplantation combined with Shenghuazhixue detection can reduce postpartum body weight, uterine wet weight and index of uterus, and promote uterine involution in female rats, which may be through decreasing the levels of intrerleukin-1 and tumor necrosis factor-α as well as increasing the levels of thromboxane B2 and vascular endothelial growth factor.

Key words: Uterine Diseases, Bone Marrow, Mesenchymal Stem Cell Transplantation, Tissue Engineering

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