中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (8): 1236-1240.doi: 10.3969/j.issn.2095-4344.2017.08.015

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

大鼠骨质疏松伴颅骨极量缺损模型的建立

黄  馗1，罗道文1，王  雷1，罗世洪1，姚志浩1，李  勇1，肖金刚1，2   

  1. 西南医科大学附属口腔医院，1口颌面修复重建和再生实验室，          2口腔颌面外科，四川省泸州市 646000
  • 收稿日期:2016-10-20 出版日期:2017-03-18 发布日期:2017-04-14
  • 通讯作者: 肖金刚，博士，教授，主任医师，硕士生导师，西南医科大学附属口腔医院，四川省泸州市 646000
  • 作者简介:黄馗，男，1990年生，汉族，西南医科大学在读硕士，主要从事口腔颌面外科临床与基础研究。
  • 基金资助:

    国家自然科学基金项目(813671125)；四川省科技厅项目(2014JY0044)；四川省教育厅项目(10-ZB030)；四川省卫生厅项目(80170)；泸州医学院重点项目(201207)；泸州市人民政府-西南医科大学科技战略合作项目(2015LZCYD-S05(2/12))

Establishing a rat model of osteoporosis with critical-size calvarial defects

Huang Kui1, Luo Dao-wen1, Wang Lei1, Luo Shi-hong1, Yao Zhi-hao1, Li Yong1, Xiao Jin-gang1, 2   

  1. 1Orofacial Reconstruction and Regeneration Laboratory, 2Department of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2016-10-20 Online:2017-03-18 Published:2017-04-14
  • Contact: Xiao Jin-gang, M.D., Professor, Chief physician, Master’s supervisor, Orofacial Reconstruction and Regeneration Laboratory, Department of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Huang Kui, Studying for master’s degree, Orofacial Reconstruction and Regeneration Laboratory, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 813671125; the Program of Sichuan Science and Technology Bureau, No. 2014JY0044; the Project of Education Department of Sichuan Province, No. 10-ZB030; the Project of Health Department of Sichuan Province, No. 80170; the Key Project of Luzhou Medical University, No. 201207; Luzhou Municipal People’s Government-the Science and Technology Strategic Cooperation Project of Southwest Medical University, No. 2015LZCYD-S05(2/12)

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被引次数

5

摘要：

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文题释义：
骨质疏松：是以骨量的减少、骨组织微细结构改变为主要特征，伴有骨脆性和骨折危险性增加的一种全身性骨骼疾病。骨量减少是指骨矿物质如钙、磷和骨基质(骨胶原)的比例降低，表现为骨强度降低，在轻微外力作用下即可发生骨折。其最常见的症状是疼痛，其中以腰背痛最为常见，其次为驼背、身长缩短，更为严重者可发生骨折，甚至导致死亡。
骨极量缺损：又称之为临界骨缺损，是指动物依靠自身修复能力终身不能恢复的大型骨缺损，其可以最大程度地减少因为动物年龄、种系、骨缺损所在部位不同等因素对骨缺损愈合的影响，排除自身干扰，建立的骨缺损动物模型更可靠。颅骨极量缺损模型常成为评价骨缺损修复作用的首选，并且Schmiz等确定大鼠颅骨临界骨缺损为8 mm。
摘要
背景：骨质疏松伴骨缺损疾病逐渐成为一个重要的社会健康问题。建立动物模型是研究该疾病的基础，且目前还没有关于建立该疾病动物模型的报道。
目的：建立骨质疏松伴颅骨极量缺损SD大鼠模型。
方法：将20只雌性SD大鼠随机分为2组，实验组行双侧卵巢切除，12周后再造8 mm颅骨极量缺损；对照组只摘除双侧卵巢周围同等质量的脂肪组织，12周后再造8 mm颅骨极量缺损。颅骨极量缺损后12周，检测离体股骨近端、胫骨远端三维骨矿含量、骨密度及血清碱性磷酸酶水平，并进行颅骨组织切片病理观察。
结果与结论：两组大鼠颅骨缺损区均未完全愈合，并且骨修复效果不明显，对照组形成新生骨量较实验组多；实验组股骨近端、胫骨远端的三维骨矿含量和骨密度均较对照组显著降低(P < 0.05)，血清碱性磷酸酶水平较对照组明显升高(P < 0.05)；与对照组比较，实验组颅骨骨小梁明显变细变小，骨髓腔明显增大，皮质骨显著变薄，新生骨基质形成明显减少。结果表表明，骨质疏松伴颅骨极量缺损SD大鼠模型建立成功。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0003-1300-7191(肖金刚)

关键词: 组织构建, 骨组织工程, 骨质疏松, 卵巢切除术, 颅骨极量缺损, 三维骨矿含量, 骨密度, 血清碱性磷酸酶, 颅骨组织切片, SD大鼠, 国家自然科学基金

Abstract:

BACKGROUND: Osteoporosis with bone defects is becoming a devastating problem. To establish an animal model is the basis of studying this disease, but how to establish the animal model is not yet reported.
OBJECTIVE: To establish a Sprague-Dawley rat model of osteoporosis accompanied with critical-size calvarial defects.
METHODS: Twenty Sprague-Dawley rats were randomly divided into two groups, followed by bilateral ovariectomy, and 8 mm critical-size calvarial defects were established 12 weeks later (experimental group) or followed by removal of the same quality of adipose tissues around bilateral ovaries and 8 mm critical-size calvarial defects were established 12 weeks later (control group). Twelve weeks after modeling, the three-dimensional bone mineral content and bone mineral density of the isolated proximal femur and distal tibial, as well as the level of serum alkaline phosphatase were detected; at the same time, the histological morphology of the skull was observed.
RESULTS AND CONCLUSION: The skull defects in both two groups were not completely healed and the repair outcome was not significant. The new bone formation in the control group was significantly more remarkable than that in the experimental group. Compared with the control group, the three-dimensional bone mineral content and bone mineral density of the proximal femur and distal tibial were significantly decreased, and the level of serum alkaline phosphatase was significantly increased in the experimental group (P < 0.05). Furthermore, in the experimental group, the trabecular bone became smaller and thinner, the bone marrow cavity was obviously increased, the cortical bone appeared to be markedly thinner, and the formation of new bone matrix was significantly decreased. To conclude, the Sprague-Dawley rat model of osteoporosis with critical-size calvarial defects is successfully established.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoporosis, Ovariectomy, Rats, Sprague-Dawely, Tissue Engineering

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