中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (5): 815-820.doi: 10.3969/j.issn.2095-4344.2017.05.026

• 干细胞综述 stem cell review • 上一篇    

诱导多能干细胞的安全性及应用研究进展

田胜男,王  博,李  琦,黄元华,马燕琳   

  1. 海南医学院第一附属医院,海南省海口市  570102
  • 出版日期:2017-02-18 发布日期:2017-02-18
  • 通讯作者: 马燕琳,博士,教授,海南医学院第一附属医院,海南省海口市 570102
  • 作者简介:Tian Sheng-nan, Master, First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China
  • 基金资助:

    国家国际科技合作专项(2014DFA30180);国家科技部973项目(2012CB966502);国家自然科学基金(81060175,30860103,81460034,81260032,81060016,31140021);海南省国际科技合作专项(GJXM20100004,GJXM201106,KJHZ2014-11);人力资源和社会保障部2011年度留学人员科技活动择优资助项目;教育部归国留学人员启动基金项目;海南医学院创新性实验项目(HYCX201304)

The safety and application of induced pluripotent stem cells

Tian Sheng-nan, Wang Bo, Li Qi, Huang Yuan-hua, Ma Yan-lin   

  1. First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China
  • Online:2017-02-18 Published:2017-02-18
  • Contact: Ma Yan-lin, M.D., Professor, First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China
  • About author:田胜男,女,1995年生,山东省菏泽市人,汉族,硕士,主要从事生殖与遗传、干细胞研究。
  • Supported by:

    the International Science & Technology Cooperation Program of China, No. 2014DFA30180; the National 973 Program of China, No. 2012CB966502; the National Natural Science Foundation of China, No. 81060175, 30860103, 81460034, 81260032, 81060016, 31140021; the International Science and Technology Cooperation Program of Hainan Province, No. GJXM20100004, GJXM201106, KJHZ2014-11; the 2011 Scientific Research Foundation for Overseas Students granted by the Human Resources and Social Security Ministry of China; the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry of China; the Innovative Experiment Project of Hainan Medical University, No. HYCX201304.

摘要:

文章快速阅读:

文题释义:
诱导多能干细胞:
多能干细胞是能够分化形成多种细胞类型的细胞。2006年,日本科学家将分化的小鼠体细胞在特定诱导因子Oct4、Sox2、c-Myc和Klf4(即OSKM体系)过表达作用下,逆转去分化重回多能干细胞,并命名为诱导多能干细胞。
诱导多能干细胞的特性:具有自我更新能力,其是指通过对称分裂产生于母代细胞完全相同子代细胞的“无限”增殖能力,也可以称之为“永生性”;具有多向分化潜能,是指在特定的诱导条件下可分化为不同表型的成熟细胞。诱导多能干细胞具有完整的基因组,即其含有来源细胞的全部遗传信息。

 

摘要
背景:
诱导多能干细胞技术解决了伦理学和免疫排斥等矛盾,高效安全定向诱导多能干细胞技术成了当今研究的主流。
目的:综述诱导多能干细胞的诱导安全性和应用进展。
方法:应用计算机检索PubMed及中国期刊全文数据库2006年1月至2016年3月有关诱导多能干细胞的文章,英文检索词为“induced pluripotent stem cell,reprogramming,clinical application,safety,transcription factor,disease model”,中文检索词为“诱导多能干细胞,重编程,临床应用,遗传安全性,转录因子,疾病模型”。
结果与结论:近年来诱导多能干细胞的研究备受科研界和医学界的关注,并努力克服了免疫和伦理问题,成功获得了诱导多能干细胞。但由于无法解决诱导多能干细胞技术中的安全性、效率和诱导多能干细胞细胞再分化机制等方面的问题,而局限于理论和实验室研究,迟迟无法应用于临床。如何安全高效的将诱导多能干细胞诱导为患者需要的类型并进行移植,如何建立良好的疾病模型与高通量药物筛选平台,涉及各方面的基础研究,面临着巨大的困难和挑战。

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID:
0000-0003-1379-0796(马燕琳)

关键词: 干细胞, 诱导, 诱导多能干细胞, 重编程, 临床应用, 遗传安全性, 转录因子, 疾病模型, 国家自然科学基金

Abstract:

BACKGROUND: Induced pluripotent stem cell technology have solved the contradiction between the ethics and immune rejection, and this high-efficient and safe technique is becoming the mainstream of today’s research.
OBJECTIVE: To comprehensively review the safety and application of induced pluripotent stem cells.
METHODS: A computer-based online retrieval of PubMed and CNKI was performed to search relevant papers published from January 2006 to April 2016, with the key words of “induced pluripotent stem cell, reprogramming, clinical application, safety, transcription factor, disease mode” in English and Chinese, respectively.
RESULTS AND CONCLUSION: In recent years, research on induced pluripotent stem cells has attracted much attention from the scientific community and the medical community, and this technique has successfully gained induced pluripotent stem cells and overcome the problems of immunity and ethics. However, it is limited to the theoretical and laboratory research due to the inability to solve the safety, efficiency and re-differentiation mechanism of induced pluripotent stem cells. Therefore, we are faced with enormous difficulties and challenges, which involve all aspects of basic research, including how to safely and effectively induce the differentiation of induced pluripotent stem cells into the desired cell type and how to establish a suitable disease model as well as a high-throughput drug screening platform.

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Stem Cells, Multipotent Stem Cells, Tissue Engineering

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