中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (1): 13-17.doi: 10.3969/j.issn.2095-4344.2017.01.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

抗坏血酸促进骨髓间充质干细胞的上皮转分化

韩效林，李敬东，王婉玲，杨  翠，李志英   

  1. 新乡医学院第一附属医院血液二科，河南省新乡市  453100
  • 修回日期:2016-11-16 出版日期:2017-01-08 发布日期:2017-03-15
  • 通讯作者: 李志英，主任医师，新乡医学院第一附属医院血液二科，河南省新乡市 453100
  • 作者简介:韩效林，女，1975年生，河北省张家口市人，汉族，1998年新乡医学院毕业，副主任医师，主要从事血液病方面的研究。
  • 基金资助:

    河南省医学科技攻关计划项目(201602152)

Epithelial transdifferentiation of bone marrow mesenchymal stem cells promoted by ascorbic acid

Han Xiao-lin, Li Jing-dong, Wang Wan-ling, Yang Cui, Li Zhi-ying   

  1. Second Department of Hematology, First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China
  • Revised:2016-11-16 Online:2017-01-08 Published:2017-03-15
  • Contact: Li Zhi-ying, Chief physician, Second Department of Hematology, First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China
  • About author:Han Xiao-lin, Associate chief physician, Second Department of Hematology, First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China
  • Supported by:

    the Medical Science Research Project of Henan Province, No. 201602152

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摘要：

文章快速阅读：

文题释义：
干细胞自稳定性：是干细胞的基本特征之一。指干细胞可在个体生命过程中自我更新并维持其自身数目恒定。干细胞的稳定有赖于细胞增殖与细胞凋亡的合理配合。任何一方的不均衡，均可引起细胞失衡，即过度增殖或过度凋亡。
干细胞的转分化：一种组织类型的干细胞在适当条件下分化为另一种组织类型的细胞的过程称干细胞的转分化。

 

摘要
背景：研究表明，抗坏血酸具有增强血管弹性、强化骨骼、抗氧化的作用。
目的：通过体外细胞分子生物学实验，观察抗坏血酸对骨髓间充质干细胞上皮转分化的促进作用。
方法：不同浓度(0，10，20，40 μmol/L)抗坏血酸处理骨髓间充质干细胞，光镜下观察骨髓间充质干细胞形态变化，筛选出最适处理浓度和时间(40 μmol/L抗坏血酸干预48 h)，进一步Realtime PCR检测骨髓间充质干细胞上皮相关分子E-钙黏蛋白、N-钙黏蛋白及间质相关分子snail1、转化生长因子β的表达，Western blot检测骨髓间充质干细胞内干性相关分子Oct4，Nanog，Sox2的表达。
结果与结论：①光镜下观察，抗坏血酸处理24 h，骨髓间充质干细胞的形态开始由长梭形逐渐向形态较圆润的上皮细胞转化。随着抗坏血酸浓度的上调，形态学变化越显著。抗坏血酸处理48 h，光镜下约有90%以上的细胞出现以上变化；②抗坏血酸可通过上调骨髓间充质干细胞上皮相关分子(N-钙黏蛋白，E-钙黏蛋白)并下调间质相关分子(snail1，转化生长因子β)的表达，促进骨髓间充质干细胞间质上皮转化；③抗坏血酸在促进骨髓间充质干细胞间质上皮转化的同时，骨髓间充质干细胞干性(Oct4，Sox2，Nanog表达)未发生明显变化。

 

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0001-9827-0604(韩效林)

关键词: 干细胞, 骨髓干细胞, 抗坏血酸, 骨髓间充质干细胞, 上皮转化

Abstract:

BACKGROUND: Ascorbic acid has been shown to promote blood vessel elasticity, strengthen bones, and exert an anti-oxidative role.
OBJECTIVE: To study the effect of ascorbic acid on the epithelial transdifferentiation of bone marrow mesenchymal stem cells in vitro by cell and molecular biology experiments.
METHODS: Different concentrations (0, 10, 20, 40 μmol/L) of ascorbic acid were used to deal with bone marrow mesenchymal stem cells. Under light microscope, we observe the morphological changes of bone marrow mesenchymal stem cells, and screened the optimal concentration of ascorbic acid (40 μmol/L) and suitable interventional time (48 hours). Further detection of E-cadherin, N-cadherin, Snail1 and transforming growth factor-β by real-time PCR was conducted. Western blot assay was also used to detect Oct4, Nanog and 
Sox2 in bone marrow mesenchymal stem cells.
RESULTS AND CONCLUSION: Under the light microscope, the long-fusiform cells began to differentiate into rounded epithelial cells after 24 hours of ascorbic acid intervention. And this change became more obvious with the increasing of concentrations. After 48 hours, approximately 90% of cells changed as described above. Ascorbic acid upregulated N-, E- cadherin and downregulated Snail1 and transforming growth factor-β, thereby promoting the epithelial transition of bone marrow mesenchymal stem cells. Meanwhile, the expression of Sox2, Nanog, and Oct4, however, did not change significantly.

 

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Ascorbic Acid, Bone Marrow, Mesenchymal Stem Cells, Epithelium, Tissue Engineering

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