中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (40): 6014-6020.doi: 10.3969/j.issn.2095-4344.2016.40.013

• 骨及关节损伤动物模型 Animal models of bone and joint injuries • 上一篇    下一篇

犬脊柱化脓性感染模型的建立

陈维华，吕国华，周 彬，康意军   

  1. 中南大学湘雅二医院脊柱外科，湖南省长沙市 410011
  • 修回日期:2016-08-10 出版日期:2016-09-30 发布日期:2016-09-30
  • 通讯作者: 康意军，博士，教授，中南大学湘雅二医院脊柱外科，湖南省长沙市 410011
  • 作者简介:陈维华，男，1977年生，辽宁省大连市人，汉族，2007年上海交通大学毕业，博士，主治医师，主要从事脊柱感染性疾病方面的研究。
  • 基金资助:

    国家自然科学基金委(30901513)

Establishment of a dog model of pyogenic spinal infection

Chen Wei-hua, Lv Guo-hua, Zhou Bin, Kang Yi-jun   

  1. Department of Spinal Surgery, Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China
  • Revised:2016-08-10 Online:2016-09-30 Published:2016-09-30
  • Contact: Kang Yi-jun, M.D., Professor, Department of Spinal Surgery, Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China
  • About author:Chen Wei-hua, M.D., Attending physician, Department of Spinal Surgery, Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30901513

PDF

243

摘要：

文章快速阅读：

 

文题释义：
脊柱化脓性感染：脊柱化脓性感染是区别于脊柱结核等疾病的脊柱非特异性感染。最常受累的部位是腰椎，其他部位依次是胸椎和颈椎，而骶椎则很少受累。男性比女性更容易赘患这种疾病，脊柱感染的诱因通常是泌尿生殖系统或身体其他部位的感染以及尿道的手术操作。
金黄色葡萄球菌：金黄色葡萄球菌隶属于葡萄球菌属，是一种革兰阳性菌。在自然界中无处不在，空气、水、灰尘及人和动物的排泄物中都可找到，是一种常见致病菌。大量文献表明金黄色葡萄球菌是脊柱化脓性感染的最常见致病菌，其所占比例可高达48%。
 
摘要
背景：脊柱感染模型在文献中鲜有报道，对脊柱感染动物模型的影像学及组织学观察报道就更少。
目的：建立一种新型的犬脊柱化脓性感染模型。
方法：取中国杂种家犬12只。动物取右侧卧位，手术入路选择腹膜后入路，手术暴露动物脊柱T12/L1椎间隙，将暴露椎间隙的椎间盘部分切除，用刮匙破坏椎间隙相邻的上下节段椎体终板，然后将含有不同浓度的金黄色葡萄球菌的悬浮液和硬化剂鱼肝油酸钠注射入犬的脊柱椎间隙。切除部分椎间盘并破坏椎体终板的目的是使椎体骨组织更好的与细菌相接触。对动物脊柱L5/6间隙完成同样操作。动物在术后14 d处死，感染的椎间盘及其相邻椎体取出，对取出标本的细菌含量进行定量测定。处死时取部分肝脏组织和抽血进行细菌培养，观测是否有全身系统性感染的发生。
结果与结论：①成功的应用金黄色葡萄球菌浓度为102 CFU/mL的细菌悬浮液在90%(9/10)的接种部位中诱导出脊柱感染。肝脏组织及血液培养结果均呈阴性，排除了全身系统性感染的可能；②接种细菌浓度高于103 CFU/mL的4只动物中有2只在术后3 d内死亡；③结果提示，成功建立了一种新型犬脊柱化脓性感染模型，这种模型可以广泛地应用于验证不同抗感染治疗方法的疗效。

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

ORCID: 0000-0002-8586-7118(陈维华)

关键词: 实验动物, 骨软骨损伤与修复动物模型, 脊柱化脓性感染, 动物模型, 犬, 金黄色葡萄球菌, 影像学观察, 组织学观察, 国家自然科学基金

Abstract:

BACKGROUND: Spine infection models are rarely reported in the literature. There are fewer reports on the imaging and histological observations of the animal model of spinal infection.

OBJECTIVE: To develop a canine model of pyogenic spinal infection.
METHODS: Twelve Chinese dogs were adopted in the study. The dogs were in the right arm recumbent. The surgery was conducted by retroperitoneal approach to expose T12/L1 intervertebral space. The intervertebral discs that exposed the intervertebral space were partially removed, and the end plate of the adjacent two vertebrae was deliberately curetted out. Suspension with different concentrations of Staphylococcus aureus and sodium morrhuate were injected into the dog intervertebral space. The aim of removal of partial intervertebral discs and destruction of endplate was to make the vertebral body bone better in contact with the bacteria. The same operation was conducted in L5/6 clearance of the animal spine. At 14 days after surgery, animals were sacrificed. The infected intervertebral disc and adjacent vertebral bodies were taken out. Bacterial contents of specimens were quantitatively determined. During sacrificing, partial liver tissues and blood samples were collected for bacterial culture. The occurrence of systemic infection was observed.
RESULTS AND CONCLUSION: (1) Spondylodiscitis of the lumbar spinal column was consistently produced in 90% (9/10) the sites challenged with 102 colony-forming units (CFU) Staphylococcus aureus. Liver biopsy and blood culture did not show any signs of systematic infections. (2) Two out of four animals implanted the suspension with a concentration higher than 103 CFUs died within 3 days post-implantation. (3) These results suggested that a new canine model of pyogenic spinal infection was developed. This model could be used to test the efficacy of different anti-infection strategies. 

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Models, Animal, Infection, Staphylococcus aureus, Tissue Engineering

中图分类号: