中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (8): 1140-1145.doi: 10.3969/j.issn.2095-4344.2016.08.011

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

重组人骨形态发生蛋白2调控腰椎融合胰岛素样生长因子Ⅰ的表达

王磊磊1，郑君涛1，胡永胜1，刘 伟1，刘 旭1，金格勒2   

  1. 1新疆医科大学第六附属医院脊柱三科，新疆维吾尔自治区乌鲁木齐市 830000；2新疆医科大学第一附属医院VIP外科，新疆维吾尔自治区乌鲁木齐市 830000
  • 收稿日期:2015-12-21 出版日期:2016-02-19 发布日期:2016-02-19
  • 通讯作者: 金格勒，教授，主任医师，新疆医科大学第一附属医院VIP外科，新疆维吾尔自治区乌鲁木齐市 830000
  • 作者简介:王磊磊，男，1984年生，山东省青岛市人，汉族，2011年新疆医科大学毕业，硕士，主治医师，主要从事非细胞型组织工程研究。

Expression of insulin-like growth factor I in the lumbar spinal fusion under control of recombinant human bone morphogenetic protein-2

Wang Lei-lei1, Zheng Jun-tao1, Hu Yong-sheng1, Liu Wei1, Liu Xu1, Jin Ge-le2   

  1. 1Third Department of Spinal Surgery, Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China; 2Department of VIP Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • Received:2015-12-21 Online:2016-02-19 Published:2016-02-19
  • Contact: Jin Ge-le, Professor, Chief physician, Department of VIP Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • About author:Wang Lei-lei, Master, Attending physician, Third Department of Spinal Surgery, Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China

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摘要：

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文题释义：

骨形态发生蛋白2：骨形态发生蛋白2是转化生长因子β超家族成员之一，是一种分泌性多功能蛋白。目前，已经发现的该超家族成员约10余种，骨形态发生蛋白合成时是一个大的前体蛋白，包括一个信号肽、一个前结构域和一个羧基末端区。羧基末端区含有100-125个氨基酸，该区域从前体蛋白上水解被释放形成二聚体，释放至细胞外，与机体各处靶细胞表面的相应受体结合而发挥作用，具有诱导未分化的间充质干细胞向成软骨细胞和成骨细胞定向分化与增殖的能力，促进成骨细胞分化成熟，参与骨和软骨的生长发育及重建过程，进而加速骨缺损的修复。骨形态发生蛋白2是目前研究最为广泛、诱导成骨活性最强的骨形态发生蛋白之一。

腰椎融合：腰椎融合是治疗腰椎疾患的一项基本技术，通过腰椎前。后路手术在椎小关节、横突间植骨或植入Cage并植骨等方法，使腰椎关节间发生骨性结合，从而建立和维持腰椎稳定性。通过植骨融合才能实现永久的稳定，获得真正意义上的稳定重建。植骨融合在重建腰椎稳定中发挥着关键的作用，融合成功与否也成为脊柱手术成功与否真要标志之一。根据融合部位，可将腰椎融合分为后侧融合、后外侧融合、椎间融合与360°融合4大类。

 

背景：骨形态发生蛋白2的成骨活性在许多实验中已被充分证实，但诱导成骨是一系列生物活性因子复杂的网络调节过程，不是单一因子的作用。
目的：定量分析重组人骨形态发生蛋白2用于兔腰椎融合过程中，胰岛素样生长因子Ⅰ基因的表达水平。
方法：将60只新西兰大白兔随机分为3组，分别在L5-6横突间植入自体骨、同种异体骨及复合骨(载有75 µg重组人骨形态发生蛋白2的同种异体骨)，植入后7，14，21，28，35 d，取L5-6横突间植骨融合所形成的骨痂，应用实时荧光定量RT-PCR检测胰岛素样生长因子Ⅰ基因的表达。
结果与结论：3组胰岛素样生长因子Ⅰ的表达均随着植入时间的延长逐渐增加，至28 d时达到高峰，随后下降。植入后7 d，自体骨组胰岛素样生长因子Ⅰ的表达高于复合骨组、异体骨组(P < 0.05)；植入后14 d，自体骨组、复合骨组胰岛素样生长因子Ⅰ的表达高于异体骨组(P < 0.05)；植入后21，28，35 d，复合骨组胰岛素样生长因子Ⅰ的表达高于自体骨组、异体骨组(P < 0.05)。结果表明重组人骨形态发生蛋白2可有效促进骨融合过程中胰岛素样生长因子Ⅰ的表达。 
ORCID: 0000-0002-8016-8772(金格勒)

关键词: 生物材料, 骨生物材料, 重组人骨形态发生蛋白2, 胰岛素样生长因子Ⅰ, 实时荧光定量反转录聚合酶链反应, 腰椎融合

Abstract:

BACKGROUND: Osteogenic ability of bone morphogenetic protein-2 has been well documented in many experiments, but a series of factors are involved in osteogenesis induction that is a complex network adjustment process.
OBJECTIVE: To quantitatively determine the level of insulin-like growth factor I during the lumbar spinal fusion of rabbits induced by recombinant human bone morphogenetic protein-2.
METHODS: Sixty adult male New Zealand white rabbits were randomly divided into three groups: bone autograft, bone allograft or composite bone (bone allograft with 75 µg recombinant human bone morphogenetic protein-2) was implanted into the L5-6 intertransverse process of rabbits, respectively. At days 7, 14, 21, 28, 35 after implantation, formed callus was taken to detect the expression of insulin-like growth factor I using real-time fluorescence quantitative PCR.
RESULTS AND CONCLUSION: In the three groups, the expression of insulin-like growth factor I gradually increased with implantation time, peaked at 28 days and then decreased. At 7 days after implantation, the expression of insulin-like growth factor I was higher in the autograft group than the composite and allograft groups (P < 0.05); at 14 days, the expression of insulin-like growth factor I was higher in the autograft and composite groups than the allograft group (P < 0.05); at 21, 28 and 35 days, the expression of insulin-like growth factor I was higher in the composite group than the autograft and allograft groups (P < 0.05). These findings indicate that recombinant human bone morphogenetic protein-2 can improve the expression of insulin-like growth factor I effectively during the lumbar spinal fusion.