中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (32): 5112-5117.doi: 10.3969/j.issn.2095-4344.2015.32.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓基质干细胞体外定向诱导后表面MHC抗原的表达

佟雁翔1，冯 卫1，苏依拉其木格2，吕慧成1，吴一民1，蒋电明3   

  1. 1内蒙古医科大学第二附属医院骨科，内蒙古自治区呼和浩特市  010030；         
    2内蒙古医科大学附属医院临床医学研究中心，内蒙古自治区呼和浩特市  010030；
    3重庆医科大学附属第一医院骨科，重庆市  400016
  • 出版日期:2015-08-06 发布日期:2015-08-06
  • 通讯作者: 吴一民，博士，硕士生导师，主任医师，教授，内蒙古医科大学第二附属医院骨科，内蒙古自治区呼和浩特市010030 并列通讯作者：蒋电明，博士生导师，教授，重庆医科大学附属第一医院骨科，重庆市 400016
  • 作者简介:佟雁翔，男，1981 年生，内蒙古自治区呼和浩特市人，汉族，重庆医科大学在读博士，主要从事骨创伤疾病、骨组织工程研究。
  • 基金资助:

    内蒙古医科大学科技百万工程项目(NY2011BW007)

MHC antigen expression on the surface of bone marrow stromal stem cells after directional induction in vitro 

Tong Yan-xiang1, Feng Wei1, Suyilaqimuge2, Lv Hui-cheng1, Wu Yi-min1, Jiang Dian-ming3   

  1. 1Department of Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; 
    2Clinical Research Center, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; 
    3Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Online:2015-08-06 Published:2015-08-06
  • Contact: Wu Yi-min, M.D., Master’s supervisor, Chief physician, Professor, Department of Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China Corresponding author: Jiang Dian-ming, Doctoral supervisor, Professor, Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, Chin
  • About author:Tong Yan-xiang, Studying for doctorate, Department of Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • Supported by:

    the Million Engineering Project for the Science and Technology of Inner Mongolia Medical University, No. NY2011BW007

PDF

565

被引次数

2

摘要：

背景：同种异体骨髓基质干细胞移植治疗骨骼疾病是研究热点，寻找有效的方法防止移植后免疫排斥反应的发生，是当前迫切需要解决的问题。
目的：探讨骨髓基质干细胞在体外诱导为成骨细胞后表面MHC抗原的表达。
方法：抽取兔骨髓组织，在体外分离、培养获得骨髓基质干细胞，加入含骨形态发生蛋白2重组蛋白的IMDM诱导培养基诱导后，流式细胞仪分析骨髓源性成骨细胞MHC抗原的表达。
结果与结论：骨髓源性成骨细胞表面MHCⅡ类抗原不表达、MHCⅠ类抗原表达。骨髓基质干细胞体外分化为成骨细胞后，未见免疫排斥反应，可用于同种异体或异种骨髓基质干细胞移植治疗骨缺损。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 干细胞, 骨髓干细胞, 骨髓基质干细胞, 分离, 培养, 分化, 成骨诱导, 成骨鉴定, MHCⅠ, MHCⅡ

Abstract:

BACKGROUND: Allogeneic bone marrow stromal stem cell transplantation for treatment of bone diseases is a hot topic. To seek effective methods for prevention of post-transplantation immune rejection is urgent to be solved.
OBJECTIVE: To explore the expression of MHC antigen on the surface of bone marrow stromal stem cells after osteogenic induction in vitro.
METHODS: Bone marrow samples were extracted from rabbits to in vitro isolate and culture bone marrow stromal stem cells. Then, the cells were cultured in IMDM medium containing bone morphogenetic protein-2. Flow cytometry was used to analyze the expression of MHC antigen on the osteoblasts differentiated from bone marrow stromal stem cells.
RESULTS AND CONCLUSION: There was highly expressed MHC I antigen but no MHCII antigen on the osteoblasts differentiated from bone marrow stromal stem cells. After osteogenic induction, no immune rejection was found. These findings indicate that allogeneic or xenogeneic bone marrow stromal cell transplantation can be used in the treatment of bone defects.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Cell Differentiation, Osteoblasts, Histocompatibility Antigens

中图分类号: