中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (40): 7539-7543.doi: 10.3969/j.issn.2095-4344.2012.40.024

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

肝癌切除肝缺血再灌注损伤前后的蛋白质组学变化

黄传钟1，林科灿2，王 斌3，黄爱民3，陈慧菁1，陶 璇3，刘景丰2，叶韵斌1   

  1. 1福建省肿瘤医院肿瘤免疫学研究室，福建省肿瘤转化医学重点实验室，福建省福州市 350014
    2福建医科大学附属第一医院肝病中心肝外科，福建省福州市 350004
    3福建医科大学基础医学院病理学系，福建省福州市 350004
  • 收稿日期:2012-01-28 修回日期:2012-03-19 出版日期:2012-09-30 发布日期:2012-09-30
  • 通讯作者: 叶韵斌，教授，主任医师，博士，福建省肿瘤医院肿瘤免疫学研究室，福建省肿瘤转化医学重点实验室，福建省福州市 350014 yunbin_ye@yahoo.com 并列通讯作者：刘景丰，教授，博士生导师，主任医师，福建医科大学附属第一医院肝病中心肝外科，福建省福州市 350004 drjingfeng@yahoo.com.cn
  • 作者简介:黄传钟★，男，1981年生，福建省福州市人，汉族，2007年厦门大学毕业，硕士，主要从事肿瘤免疫学与蛋白质组学方面的研究。 Hcz_2001@sina.com

Differential proteomic research of liver ischemia-reperfusion injury undergoing resection of hepatocellular carcinomas

Huang Chuan-zhong1, Lin Ke-can2, Wang Bin3, Huang Ai-min3, Chen Hui-jing1, Tao Xuan3, Liu Jing-feng2, Ye Yun-bin1   

  1. 1Fujian Provincial Key Laboratory of Tumor Translational Medicine, Laboratory of Tumor Immunology, Fujian Provincial Tumor Hospital, Fuzhou 350014, Fujian Province, China
    2Department of Liver Surgery, Liver Center of the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China
    3Department of Pathology, School of Basic Medical Science, Fujian Medical University, Fuzhou 350004, Fujian Province, China
  • Received:2012-01-28 Revised:2012-03-19 Online:2012-09-30 Published:2012-09-30
  • Contact: Ye Yun-bin, Doctor, Professor, Chief physician, Fujian Provincial Key Laboratory of Tumor Translational Medicine, Laboratory of Tumor Immunology, Fujian Provincial Tumor Hospital, Fuzhou 350014, Fujian Province, China yunbin_ye@yahoo.com Liu Jing-feng, Professor, Doctoral supervisor, Chief physician, Department of Liver Surgery, Liver Center of the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China drjingfeng@yahoo.com.cn
  • About author:Huang Chuan-zhong★, Master, Fujian Provincial Key Laboratory of Tumor Translational Medicine, Laboratory of Tumor Immunology, Fujian Provincial Tumor Hospital, Fuzhou 350014, Fujian Province, China Hcz_2001@sina.com

PDF

568

被引次数

9

摘要：

背景：肝癌切除肝缺血再灌注损伤可促进微小转移灶的侵袭转移并引起线粒体损伤。
目的：比较肝癌切除手术过程中肝正常组织缺血再灌注损伤前后蛋白质表达谱的改变，筛查发生肝缺血再灌注损伤应激的相关蛋白质分子标志物并探讨可能机制。
方法：应用双向等电聚焦/聚丙烯酰胺凝胶电泳建立20例肝癌切除患者肝缺血再灌注前与灌注后15 min肝正常组织的全细胞蛋白质和线粒体蛋白质表达图谱，并进行差异分析，应用基质辅助激光解吸离子化串联时间质谱对差异蛋白质点进行鉴定，并进行生物信息学分析。
结果与结论：相同条件下对各组全细胞和线粒体蛋白样品分别进行3次双向电泳，筛选在3次电泳中均存在的差异点进行分析，在全细胞蛋白质图谱中共鉴定出5个差异蛋白，在线粒体蛋白质图谱中未鉴定出明显的差异蛋白。结果可见肝癌切除手术过程中肝缺血再灌注15 min即可使细胞骨架蛋白、热休克蛋白等细胞保护蛋白表达发生改变，同时有促进肿瘤转移的风险，需要采取预防措施降低影响，但线粒体蛋白表达基本未发生改变。

关键词: 肝癌, 缺血再灌注, 蛋白质组, 线粒体, 双向凝胶电泳, 透射电镜

Abstract:

BACKGROUND: The ischemia reperfusion injury undergoes resection of hepatocellular carcinomas may promote the occult metastatic invasion and metastasis, and induce mitochondrial damage.
OBJECTIVE: To reveal the protein expression changes of normal liver tissue after ischemia reperfusion injury undergoes resection of hepatocellular carcinomas, and to screen the hepatic ischemia reperfusion injury stress related protein and to explore the possible mechanism.
METHODS: Normal liver tissue proteins and mitochondrial protein expression profiles were established in 20 liver cancer patients with hepatic ischemia reperfusion before and after perfusion for 15 minutes by isoelectric focusing/polyacrylamide gel electrophoresis, and the difference was analyzed. The matrix-assisted laser desorption inoization-time of flight mass spectrometry was used to identified different expression proteins, and the bioinformatic analysis was performed.
RESULTS AND CONCLUSION: Under the same condition, two-dimensional electrophoresis of each whole cell and mitochondrial protein samples were performed for three times. Different proteins appeared in each pair profile were analyzed. In the whole cell protein profiles, a total of 5 different proteins were identified and no significant difference of protein in mitochondrial profiles was identified. Liver ischemia reperfusion 15 minutes during hepatocellular carcinoma resection operation can change the expression of the cell protective protein such as cytoskeletal proteins and heat shock proteins, and can promote tumor metastasis risk and the preventive measures should be taken to reduce the impact. However, no changes happen in mitochondrial.

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