中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (31): 5763-5767.doi: 10.3969/j.issn.2095-4344.2012.31.015

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

兔角膜移植后房水中血管内皮生长因子及肿瘤坏死因子α表达与 高压氧的影响

谢迎宾，王 强，雷宁玉，张 磊，任 莉   

  1. 滨州医学院附属医院眼科，山东省滨州市 256603
  • 收稿日期:2011-12-07 修回日期:2012-01-28 出版日期:2012-07-29 发布日期:2012-07-29
  • 作者简介:谢迎宾★，男，1973年生，山东省滨州市人，2008年青岛大学毕业，硕士，主治医师，主要从事角膜病、白内障方面的研究。 xieyingbin0529@163.com

Effects of hyperbaric oxygen on the expression of vascular endothelial growth factor and tumor necrosis factor alpha in the aqueous humour after penetrating keratoplasty in rabbits

Xie Ying-bin, Wang Qiang, Lei Ning-yu, Zhang Lei, Ren Li   

  1. Department of Ophthalmology, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China
  • Received:2011-12-07 Revised:2012-01-28 Online:2012-07-29 Published:2012-07-29
  • About author:Xie Ying-bin★, Master, Attending physician, Department of Ophthalmology, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China] xieyingbin0529@163.com

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摘要：

背景：血管内皮生长因子和肿瘤坏死因子α在角膜移植免疫反应中起重要作用，但二者与高压氧之间的关系尚不明确。
目的：探讨高压氧对角膜移植后房水中血管内皮生长因子及肿瘤坏死因子α表达的影响及其与角膜移植免疫反应的关系。
方法：分别以鸡、兔为供、受体建立异种异体穿透性角膜移植模型。将健康新西兰白兔16只随机分为2组，高压氧组从手术当日至术后第6天行高压氧治疗，对照组未行高压氧治疗。分别于角膜移植后第3，7，14，28天抽取房水，应用ELISA双抗体夹心法检测房水中肿瘤坏死因子α及血管内皮生长因子水平的变化。
结果与结论：正常兔眼房水中均可检测到少量的肿瘤坏死因子α和血管内皮生长因子。移植后早期两组房水中肿瘤坏死因子α和血管内皮生长因子水平即升高，与移植前比差异均有显著性意义(P < 0.05)。在移植后14 d左右急性排斥反应期，肿瘤坏死因子α和血管内皮生长因子达最高值。高压氧组中肿瘤坏死因子α和血管内皮生长因子的水平均明显低于对照组(P < 0.05)。结果表明高压氧可通过降低房水中肿瘤坏死因子α和血管内皮生长因子的含量，抑制角膜移植后的免疫反应。

关键词: 血管内皮生长因子, 肿瘤坏死因子α, 角膜移植, 免疫排斥, 高压氧

Abstract:

BACKGROUND: Studies had demonstrated that vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNF-α) may play an important role in immunologic rejection after penetrating keratoplasty, but the interactions between hyperbaric oxygen, VEGF and TNF-α remains unclear．
OBJECTIVE: To investigate the effects of hyperbaric oxygen on the expression of VEGF and TNF-α in the aqueous humour after penetrating keratoplasty, and to study the relationship between VEGF, TNF-α and cornea transplantation immunologic rejection.
METHODS: We used the chicken to rabbit model of penetrating heterograft. Sixteen healthy New Zealand rabbits were randomly divided into two groups: control group and hyperbaric oxygen group. The rabbits in the hyperbaric oxygen group were treated with hyperbaric oxygen from the 1st day to the 6th day after operation, and the rabbits in the control group were not treated with hyperbaric oxygen. The level of VEGF and TNF-α were determined dynamically by enzyme linked immunosorbent assay on 3, 7, 14, and 28 days after surgery.
RESULTS AND CONCLUSION: The TNF-α and VEGF expression could be seen in the aqueous humour of normal rabbits, and the levels were increased in early postoperative days, which had a significant difference statistically before and after surgery (P < 0.05). In the acute rejection period, at 14 days after transplantation, the quantity of TNF-α and VEGF reached their peak respectively, The content of TNF-α and VEGF in the hyperbaric oxygen group were obviously lower than those in the control group (P < 0.05). Hyperbaric oxygen can effectively reduce the level of VEGF and TNF-α and inhibit the immune response after keratoplasty.

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