中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (21): 3418-3425.doi: 10.3969/j.issn.2095-4344.1764

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

异基因造血干细胞移植后发生移植物抗宿主病患者血清外泌体miRNAs敏感生物标志物的表达分析

刘 娇1,王大明1,安泰学2,胡雪松2,李南凯2,汪洪富1,马 雯1,聂赫中容1,肖利佳1,周义文1,郑 磊2   

  1. 1南方医科大学深圳医院,广东省深圳市 518100;2南方医科大学南方医院,广东省广州市 510510
  • 修回日期:2019-03-26 出版日期:2019-07-28 发布日期:2019-07-28
  • 通讯作者: 王大明,副主任技师,南方医科大学深圳医院,广东省深圳市 518100
  • 作者简介:刘娇,女,1984年生,江西省萍乡市人,汉族,2009年中南大学湘雅医学院毕业,主管检验师,主要从事分子诊断研究。
  • 基金资助:

    广东省自然科学基金项目(2015A030313825),项目负责人:王大明;广东省医学科研基金青年基金(A2017143),项目负责人:马雯;深圳市科技创新委员会基础研究项目(JCYJ20170307174738825),项目负责人:王大明;深圳市卫生计生系统科研项目(201506076),项目负责人:邹红岩;深圳市卫生计生系统科研项目(201601040),项目负责人:马雯;深圳市卫生计生系统科研项目(SZBC2017014),项目负责人:聂赫中容;深圳市宝安区医疗卫生基础研究项目(2017JD184),项目负责人:聂赫中容;深圳市宝安区医疗卫生基础研究项目(2018JD122),项目负责人:刘娇

Expression profile analysis of miRNAs in serumal exosomes as sensitive biomarkers in patients with graft-versus-host disease following allogeneic hematopoietic stem cell transplantation

Liu Jiao1, Wang Daming1, An Taixue2, Hu Xuesong2, Li Nankai2, Wang Hongfu1, Ma Wen1, Nie-He Zhongrong1, Xiao Lijia1, Zhou Yiwen1, Zheng Lei2   

  1. 1Shenzhen Hospital, Southern Medical University, Shenzhen 518100, Guangdong Province, China; 2Nanfang Hospital, Southern Medical University, Guangzhou 510510, Guangdong Province, China
  • Revised:2019-03-26 Online:2019-07-28 Published:2019-07-28
  • Contact: Wang Daming, Associate chief technician, Shenzhen Hospital, Southern Medical University, Shenzhen 518100, Guangdong Province, China
  • About author:Liu Jiao, Laboratorian in charge, Shenzhen Hospital, Southern Medical University, Shenzhen 518100, Guangdong Province, China
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 2015A030313825 (to WDM); the Guangdong Provincial Medical Research Foundation for the Youth, No. A2017143 (to MW); Basic Research Project of Shenzhen Science and Technology Innovation Committee, No. JCYJ20170307174738825 (to WDM); Shenzhen Health and Family Planning System Research Projects, No. 201506076 (to ZHY), 201601040 (to MW) and SZBC2017014 (to NHZR); Medical and Health Basic Research Project of Shenzhen Baoan District, No. 2017JD184 (to NHZR) and 2018JD122 (to LJ)

摘要:

文章快速阅读:

文题释义:
外泌体:
是一类介导体内生物信息分子传递的纳米级小囊泡,由Johnstone等于1987年提出。外泌体能在体内运载和传递生物信息分子,在免疫调节、心血管疾病和肿瘤等生理病理过程中发挥着重要的功能。
急性移植物抗宿主病:是异基因造血干细胞移植后常见的并发症,移植后约有50%的患者会发生急性移植物抗宿主病,通常以皮肤、肠道、肝脏等脏器损害为主,严重者可导致死亡。

 

摘要
背景:
当前国内外关于人类造血干细胞移植后外泌体miRNA和移植物抗宿主病发生的相关性研究甚少。国内研究匮乏,外泌体来源的核酸分子有望成为新型生物标志物预测急性移植物抗宿主病的发生。
目的:探讨异基因造血干细胞移植后发生急性移植物抗宿主病患者外周血血清外泌体中候选基因miRNAs的差异表达。
方法:收集2017年8月至2018年5月异基因造血干细胞移植后发生急性移植物抗宿主病以及未发生急性移植物抗宿主病患者共30例,其中急性移植物抗宿主病组20例,非急性移植物抗宿主病组10例。提取移植前后血清外泌体及miRNA,基因芯片杂交后图像采集和数据分析。
结果与结论:与对照组相比,移植前后呈上升或下降趋势且信号差异倍数超过10倍的miRNAs在急性移植物抗宿主病组有11个miRNAs上升,26个下降。hsa-miR-3976、hsa-miR-122-5p、hsa-miR-3125明显上调,hsa-miR-941、hsa-miR-4687-5P和hsa-miR-4769-5p明显下调。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID:
0000-0002-3848-3779(王大明)

关键词: 异基因造血干细胞移植, 急性移植物抗宿主病, 外泌体, miRNA, 基因芯片, 生物标志物, 广东省自然科学基金

Abstract:

BACKGROUND: There are few studies, especially in China, addressing the correlation between exosome miRNAs and graft-versus-host disease (GVHD) following human hematopoietic stem cell transplantation. Exosome-derived nucleic acid molecules are expected to become new biomarkers for predicting the occurrence of acute GVHD (aGVHD).
OBJECTIVE: To investigate the differential expression of candidate gene microRNAs in peripheral blood serum exosomes of aGVHD patients with after allogeneic hematopoietic stem cell transplantation. 
METHODS: A total of 30 patients with aGVHD and without aGVHD after allogeneic hematopoietic stem cell transplantation from August 2017 to May 2018 were enrolled, including 20 in aGVHD group and 10 in non-aGVHD group. Serum exosomes and miRNAs were extracted before and after transplantation. Image acquisition and data analysis were performed after gene chip hybridization. 
RESULTS AND CONCLUSION: After microarray hybridization microRNAs showed an over 10-fold upward or downward trend before and after transplantation. Compared with the control group, 11 microRNAs increased and 26 decreased in the aGVHD group. hsa-miR-3976, hsa-miR-122-5p, and hsa-miR-3125 were significantly up-regulated, while hsa-miR-941, hsa-miR-4687-5P and hsa-miR-4769-5p were significantly down-regulated.

Key words: allogeneic hematopoietic stem cell transplantation, acute graft-versus-host disease, exosomes, miRNA, gene chip, biomarker, Natural Science Foundation of Guangdong Province

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