中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (25): 4627-4631.doi: 10.3969/j.issn.1673-8225.2012.25.014

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

精氨酸甘氨酸天冬氨酸多肽表面修饰促进生物支架材料对骨髓来源 种子细胞的黏附

李 伟，徐更强，周华松，韩海霞   

  1. 炮兵指挥学院廊坊校区门诊部，河北省廊坊市 065000
  • 收稿日期:2011-11-23 修回日期:2011-12-23 出版日期:2012-06-17 发布日期:2013-11-04
  • 作者简介:李伟，男，1977年生，河北省廊坊市人，汉族，1999年解放军第四军医大学临床系毕业，主治医师，主要从事心血管病防治的研究。 Windzs115420@yahoo.com.cn

Promoted effect of surface modification with arginine-glycine-aspartic acid polypeptides on the adhesion of bone marrow stem cells to biological scaffolds

Li Wei, Xu Geng-qiang, Zhou Hua-song, Han Hai-xia   

  1. Outpatient Department, Langfang School Campus of Artilleryman Command College, Langfang 065000, Hebei Province, China
  • Received:2011-11-23 Revised:2011-12-23 Online:2012-06-17 Published:2013-11-04
  • About author:Li Wei, Attending physician, Outpatient Department, Langfang School Campus of Artilleryman Command College, Langfang 065000, Hebei Province, China Windzs115420@ yahoo.com.cn

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摘要：

背景：精氨酸甘氨酸天冬氨酸多肽具有较强的黏附性和生物支架材料可接枝结合，且不会改变材料的表面理化性质。
目的：观察应用精氨酸甘氨酸天冬氨酸多肽表面修饰猪主动脉瓣去细胞支架材料对骨髓干细胞黏附性的影响。
方法：采用胰蛋白酶+TritonX-100法制备猪主动脉瓣去细胞支架材料，用YGRGDSP多肽(酪氨酸-甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸-脯氨酸)进行处理，按照精氨酸甘氨酸天冬氨酸多肽的质量浓度(0.5，1.0，1.5，2.0 g/L)、反应时间(4，8，12，24 h)、反应pH值(7.0，7.4，8.0)分为不同实验组。
结果与结论：茚三酮显示精氨酸甘氨酸天冬氨酸多肽可很好的交联到猪主动脉瓣去细胞支架材料，最佳反应条件为：室温、1.5 g/L精氨酸甘氨酸天冬氨酸、pH 7.4、持续振荡12 h。提示利用YGRGDSP多肽对猪主动脉瓣去细胞支架材料进行表面修饰可显著改善骨髓来源种子细胞的黏附性。

关键词: 精氨酸甘氨酸天冬氨酸多肽, 骨髓间充质干细胞, 去细胞瓣膜, 表面修饰, 细胞黏附, 生物材料

Abstract:

BACKGROUND: Arginine-glycine-aspartic acid (RGD) has strong adhesion, and can graft with biological scaffold materials without changing surface physical and chemical properties of the materials.
OBJECTIVE: To observe the effect of surface modification with RGD polypeptides on the adhesion of bone marrow stem cells (BMSCs) to biological scaffolds.
METHODS: Cells and cellular components were removed from porcine aortic valve by using trypsin and hyposmosis TritonX-100 methods, and then modified with tyrosine-glycine-arginine-glycine-aspartic acid-serine-proline peptides. Decellularized valve scaffolds were divided into different groups according to the concentration of RGD peptides (0.5, 1.0, 1.5, 2.0 g/L), reaction times (4, 8, 12, 24 hours) and reaction pH values (7.0, 7.4, 8.0).
RESULTS AND CONCLUSION: The results of ninhydrin reaction showed cells were prone to attach the decellularized valve scaffolds coupled with RGD peptides. Optimum reaction conditions were at room temperature, the concentration of RGD peptides was 1.5 g/L, PH was 7.4, and sustained oscillation for 12 hours. It is indicated that decellularized valve scaffold modified with tyrosine-glycine-arginine-glycine-aspartic acid-serine-proline peptides can significantly improve the BMSCs adhesion.

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