中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (12): 2129-2132.doi: 10.3969/j.issn.1673-8225.2012.12.009

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米羟基磷灰石复合骨形态发生蛋白2与骨髓基质干细胞的体外培养*☆

代  平1，张  洪2，陈新来1，袁建辉3   

  1. 1深圳市宝安区福永人民医院骨科，广东省深圳市  518103；2深圳市新建市属医院筹备办公室，广东省深圳市  518020；3广东省深圳市疾病预防控制中心，广东省深圳市  518000
  • 收稿日期:2011-08-22 修回日期:2011-10-22 出版日期:2012-03-18 发布日期:2012-03-18
  • 作者简介:代平☆，男，1971年生，湖南省南县人，汉族，中南大学湘雅医学院在读博士，副主任医师，主要从事创伤、脊柱外科研究。

Nano-hydroxyapatite/bone morphogenetic protein-2 culture with bone marrow stem cells in vitro*☆

Dai Ping1, Zhang Hong2, Chen Xing-lai1, Yuan Jian-hui3   

  1. 1Department of Orthopedics, Fuyong People's Hospital of Baoan District, Shenzhen  518103, Guangdong Province, China;    2Shenzhen Health Bureau New Hospitals Preparatory Office, Shenzhen  518020, Guangdong Province, China;    3Shenzhen Center for Disease Control and Prevention, Shenzhen  518000, Guangdong Province, China
  • Received:2011-08-22 Revised:2011-10-22 Online:2012-03-18 Published:2012-03-18
  • About author:Dai Ping☆, Studying for doctorate, Associate chief physician, Department of Orthopedics, Fuyong People's Hospital of Baoan District, Shenzhen 518103, Guangdong Province, China dpqiangtongding@ yahoo.com.cn

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摘要：

背景：纳米级羟基磷灰石支架材料复合骨形态发生蛋白2因子组织构建模式组合后对骨髓基质干细胞的作用是叠加促进或抑制作用以及其生物相容性如何？
目的：验证纳米羟基磷灰石复合骨形态发生蛋白2与骨髓基质干细胞在体外共同培养下的生物相容性和成骨性。
方法：将兔骨髓基质干细胞体外培养、传代和扩增,经鉴定后将第3代细胞分别接种在对照组、骨形态发生蛋白2组、纳米羟基磷灰石组、纳米羟基磷灰石/骨形态发生蛋白2组4种培养液中。
结果与结论：对照组和纳米羟基磷灰石组的细胞倍增时间较骨形态发生蛋白2组、纳米羟基磷灰石/骨形态发生蛋白2组两组长；骨形态发生蛋白2组和纳米羟基磷灰石/骨形态发生蛋白2组细胞增殖能力及碱性磷酸酶测量值明显大于对照组和纳米羟基磷灰石组(P < 0.01)。提示纳米羟基磷灰石复合骨形态发生蛋白2的组织构架不仅与骨髓基质干细胞之间具有良好的生物相容性,还可发挥骨形态发生蛋白2促进骨髓基质干细胞增殖和定向分化成骨的作用。
关键词：纳米羟基磷灰石；骨形态发生蛋白2；骨髓基质干细胞；生物相容性；支架；碱性磷酸酶
doi:10.3969/j.issn.1673-8225.2012.12.009
中图分类号: R318  文献标识码: B  

关键词: 纳米羟基磷灰石, 骨形态发生蛋白2, 骨髓基质干细胞, 生物相容性, 支架, 碱性磷酸酶

Abstract:

BACKGROUND: Whether a combination of the nano-hydroxyapatite (nHA) scaffolds with bone morphogenetic protein-2 (BMP-2) is superimposed to promote or inhibit the bone marrow stromal stem cells, as well as its biocompatibility, remain unclear.
OBJECTIVE: To observe the biocompatibility and bone formation activity of nHA/BMP-2 co-cultured with rabbit bone marrow stem cells in vitro.
METHODS: Bone marrow stem cells obtained from rabbits were cultured and proliferated in DMEM medium in vitro. The cells at the third passage were inoculated into four mediums and according divided into four groups: control group, BMP-2 group, nHa group, and nHa/BMP-2 group.
RESULTS AND CONCLUSION: The cell diplo-proliferation time of control group and nHa group was longer than that in BMP-2 group and nHa/BMP-2 group. The cells proliferative activity calculated by MTT assay and bone formation activity based on the alkaline phosphatase method demonstrated statistical significant difference in BMP-2 group and nHa/BMP-2 group compared with control and nHa group (P < 0.01). Experimental findings indicate that, the tissue formation of nHA/BMP-2 not only has good biocompatibility with Bone marrow stem cells, but also could bring into full play the effect of BMP-2 for promoting the proliferation, differentiation, and bone formation activity of bone marrow stem cells.

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