中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9561-9564.doi: 10.3969/j.issn.1673-8225.2011.51.015

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

胶原蛋白-壳聚糖复合纳米纤维膜用于皮肤缺损的修复

余丕军1,2，王露萍3，郭  妤3，陈  炜3，莫秀梅4   

  1. 1江苏大学临床医学院，江苏省镇江市  212013
    2上海交通大学医学院附属第九人民医院麻醉科，上海市  200011
    3上海市第八人民医院，上海市  200235
    4东华大学化工与生物工程学院，上海市  201620
  • 收稿日期:2011-07-14 修回日期:2011-10-21 出版日期:2011-12-17 发布日期:2011-12-17
  • 通讯作者: 陈炜，教授，主任医师，上海市第八人民医院，上海市 200235 drchenwei@163.com 通讯作者：莫秀梅，教授，东华大学化工与生物工程学院，上海市 201620 xmm@dhu.edu.cn
  • 作者简介:余丕军★，男，1982年生，四川省广元市人，汉族，2011年江苏大学毕业，硕士，医师，主要从事整形外科学、皮肤组织工程的研究。 yupeijun82@ 163.com
  • 基金资助:

    国家科技部863科研基金资助项目(2008AA03Z305)。 

Repair of skin defect with collagen-chitosan compound nanofiber membrane

Yu Pei-jun1, 2, Wang Lu-ping3, Guo Yu3, Chen Wei3, Mo Xiu-mei4   

  1. 1College of Clinical Medicine, Jiangsu University, Zhenjiang  212013, Jiangsu Province, China
    2Department of Anesthesiology, the Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200011, China
    3the Eighth People’s Hospital of Shanghai, Shanghai  200235, China
    4College of Chemical Engineering and Biological Engineering, Donghua University, Shanghai  201620, China
  • Received:2011-07-14 Revised:2011-10-21 Online:2011-12-17 Published:2011-12-17
  • Contact: Chen Wei, Professor, Chief physician, the Eighth People’s Hospital of Shanghai, Shanghai 200235, China drchenwei@163.com Correspondence to: Mo Xiu-mei, Professor, College of Chemical Engineering and Biological Engineering, Donghua University, Shanghai 201620, China xmm@dhu.edu.cn
  • About author:Yu Pei-jun★, Master Physician, College of Clinical Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China; Department of Anesthesiology, the Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China yupeijun82@163.com
  • Supported by:

    National High Technology Research and Development Program of China, No. 2008AA03Z305*

PDF

443

被引次数

14

摘要：

背景：体外实验证实：胶原蛋白-壳聚糖(80∶20)复合纳米纤维膜具有优异的生物力学性能、生物相容性和生物可降解性。但目前缺乏此材料在体内方面的研究。
目的：观察胶原蛋白-壳聚糖复合纳米纤维膜修复SD大鼠背部全层皮肤缺损创面的作用。
方法：30只SD大鼠制备背部全层皮肤缺损创面后随机抽签法分为2组，实验组用胶原蛋白-壳聚糖(80∶20)复合纳米纤维膜覆盖后，用油纱及干纱布包扎并创缘外缝线打包固定；对照组仅用油纱及干纱布包扎并创缘外打包固定。
结果与结论：修复后14 d实验组创面已基本对合，干痂；苏木精-伊红染色显示创面毛细血管数量减少，纤维含量增多。对照组创面对合不整齐，创面较实验组大；苏木精-伊红染色显示毛细血管数量较多，扩张明显，大量炎性细胞浸润。提示胶原蛋白-壳聚糖复合纳米纤维膜较普通纱布敷料能更好的促进创伤修复、愈合。

关键词: 胶原蛋白, 壳聚糖, 复合纳米纤维膜, 皮肤缺损, 创伤修复

Abstract:

BACKGROUND: Studies in vitro have confirmed that collagen-chitosan (80:20) compound nanofiber membranes have excellent mechanical properties, biocompatibility and biodegradability. However, there are few researches in vivo about the materials.
OBJECTIVE: To observe the effect of collagen-chitosan compound nanofiber membranes on repair of full-thickness skin defect on the back of Sprague Dawley (SD) rats.
METHODS: Thirty SD rats with full-thickness skin defects on the back were divided into two groups randomly by lottery method. SD rats treated with collogen-chitosan (80:20) compound nanofiber membrane and oily gauzes on the defects were taken as the experimental group, while those treated with oily gauzes only as the control group. The defects of all rats were fixed with dry gauze bandage at the margin of outer packaging.
RESULTS AND CONCLUSION: The wounds healed basically in the experimental group at 14 days after the repair; hematoxylin-eosin staining showed that the numbers of capillaries were reduced and the content of fibers was increased. While in the control group, wound healed irregularly, and the wound was larger than that in the experimental group; hematoxylin-eosin staining showed that there were more obvious dilated capillaries and a large number of inflammatory cells. The above results indicated that collagen-chitosan compound nanofiber membrane is superior to ordinary gauze in promoting the tissue repair and wound healing of skin defects.

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