中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9505-9508.doi: 10.3969/j.issn.1673-8225.2011.51.002

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

富血小板血浆与脱蛋白骨复合物能增强早期腱-骨界面的愈合

翟文亮1，吕辰玮2，丁真奇1，高跃川2，郑燕梅2   

  1. 1厦门大学附属东南医院骨科，福建省漳州市 363000
    2福建中医药大学骨伤科，福建省福州市 350108
  • 收稿日期:2011-09-28 修回日期:2011-11-05 出版日期:2011-12-17 发布日期:2011-12-17
  • 作者简介:翟文亮★，男，1971年生，山东省日照市人，汉族，1997年解放军第一军医大学毕业，硕士，副主任医师，副教授，硕士生导师，主要从事骨与关节外科研究。 wlzhai@263.net
  • 基金资助:

    南京军区医学科技创新课题基金资助项目(09MA070)，课题名称：富血小板血浆促进前交叉韧带重建术后腱骨愈合的实验研究。

Platelet-rich plasma combined with deproteinized bone promotes healing of tendon-bone interface at early stage

Zhai Wen-liang1, Lü Chen-wei2, Ding Zhen-qi1, Gao Yue-chuan2, Zheng Yan-mei2   

  1. 1Department of Orthopedics, the 175 Hospital of Chinese PLA, Xiamen University, Zhangzhou  363000, Fujian Province, China
    2Department of Orthopedics, Fujian University of Traditional Chinese Medicine, Fuzhou  350108, Fujian Province, China
  • Received:2011-09-28 Revised:2011-11-05 Online:2011-12-17 Published:2011-12-17
  • About author:Zhai Wen-liang★, Master, Associate chief physician, Associate professor, Master’s supervisor, Department of Orthopedics, the 175 Hospital of Chinese PLA, Xiamen University, Zhangzhou 363000, Fujian Province, China wlzhai@263.net
  • Supported by:

    Foundation of Medical Technology Innovation of Nanjing Military Region, No. 09MA070*

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433

被引次数

3

摘要：

背景：单独使用富血小板血浆凝胶已被证实并不能提高骨再生能力，且容易在关节液破坏下迅速流失或失活。
目的：观察富血小板血浆与脱蛋白骨复合物对前交叉韧带重建后早期腱-骨界面的生物力学性能的影响。
方法：将新西兰大白兔分为正常组、模型组、实验组和对照组，后3组建立自体肌腱重建膝前交叉韧带模型，模型组骨隧道中不植入任何材料，对照组和实验组分别植入脱蛋白骨与富血小板血浆+脱蛋白骨复合物。
结果与结论：治疗后2，4周时，各组拉力卸载方式主要是移植肌腱从骨隧道中拔出；8周时，实验组以肌腱体部撕裂为主，对照组与模型组大部分仍从骨隧道拔出，差异有显著性意义(P < 0.05)；12周时，各组多数见肌腱体部撕裂。治疗后4，8周时，实验组最大抗拉载荷明显高于对照组及模型组(P < 0.05)；12周时，各组最大抗拉载荷差异无显著性意义(P > 0.05)；12周时，实验组最大抗拉载荷仍明显小于正常组(P < 0.01)。各组刚度随时间变化呈逐渐增大的趋势，但组间比较差异无显著性意义(P > 0.05)。 说明富血小板血浆与脱蛋白骨复合物能够增加早期腱-骨界面的力学强度。

关键词: 脱蛋白骨, 富血小板血浆, 前交叉韧带, 腱-骨愈合, 生物力学, 重建

Abstract:

BACKGROUND: Studies show that the platelet-rich plasma (PRP) gel cannot improve the regeneration of bone and it may be lost or inactivated rapidly by synovial fluid.
OBJECTIVE: To observe the effects of PRP combined with deproteinized bone (DPB) on the biomechanical property of tendon-bond interface at the early stage after reconstruction of the anterior cruciate ligament (ACL).
METHODS: New Zealand white rabbits were divided into normal group, model group, experimental group, and control group. The later three groups were used to prepare ACL reconstruction model. Nothing was implanted into the bone tunnel of the model group while DPB and PRP+DPB were implanted into the control group and experimental group respectively.
RESULTS AND CONCLUSION: At 2 and 4 weeks after surgery, the mainly tensile unload way in each group was that the transplanted tendon was extracted from the bone tunnel. At 8 weeks, the way mainly in the experimental group was tendon tearing, while for the control group and model group, majority of the transplanted tendons were still extracted from the bone tunnels. The differences had statistical significance (P < 0.05). At 12 weeks, tendon tearing was the main reason of tensile unloading for all of the groups. The maximum tensile load in the experimental group was obviously higher than those of the control and model groups at 4 and 8 weeks after surgery (P < 0.05); the differences had no significance at 12 weeks (P > 0.05), however, the difference between the experimental group and the normal group was still significant (P < 0.01). The difference in stiffness of each group was increased as time went on, but the differences between each group were not significant (P > 0.05). PRP+DPB can reinforce the biomechanics strength of the tendon-bond interface in the early stage.

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