中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9496-9500.doi: 10.3969/j.issn.1673-8225.2011.50.045

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    

内脏脂肪组织FOXC2 mRNA表达水平与2型糖尿病的关系

念  馨1，卫俊杰2，苏艳丹3，刘  华3，张旭祥1，李  红1   

  1. 昆明医学院第一附属医院，1内分泌科，3检验科临床基因实验室，云南省昆明市   650032； 2云南省中医院检验科，云南省昆明市  650021
  • 收稿日期:2011-06-03 修回日期:2011-10-19 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 苏艳丹，主管技师，昆明医学院第一附属医院检验科临床基因实验室，云南省昆明市 650032 lh197306@sohu.com
  • 作者简介:念馨★，女，1975年生，云南省昆明市人，汉族，硕士，主治医师，主要从事糖尿病及其并发症研究。 nianxinkm@hotmail.com
  • 基金资助:

    2005云南省教育厅项目(5y0336)，《FOXC2基因变异与代谢综合征相关性研究》。

Relations between the expression level of FOXC2 mRNA in ventral adipose tissue and type 2 diabetes

Nian Xin1, Wei Jun-jie2, Su Yan-dan3, Liu Hua3, Zhang Xu-xiang1, Li Hong1   

  1. 1Department of Endocrinology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China; 2Department of Inspection, Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming 650021, Yunnan Province, China; 3Clinical Genetic Laboratory, Department of Inspection, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • Received:2011-06-03 Revised:2011-10-19 Online:2011-12-10 Published:2011-12-10
  • Contact: Su Yan-dan, Technician-in-charge, Clinical Genetic Laboratory, Department of Inspection, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China lh197306@sohu.com
  • About author:Nian Xin★, Master, Attending physician, Department of Endocrinology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China nianxinkm@hotmail.com
  • Supported by:

     the Education Department of Yunnan Province in 2005, No. 5y0336*

PDF

417

被引次数

4

摘要：

背景：FOXC2在能量代谢等方面发挥重要作用，与脂肪细胞分化密切相关，且FOXC2基因在抵御2型糖尿病及代谢综合征相关因素方面有重要作用。
目的：检测云南地区人群大网膜脂肪组织中FOXC2 mRNA表达水平，探讨其与2型糖尿病的关系。
方法：随机抽取在昆明医学院附属医院行腹部外科手术的2型糖尿病患者及非2型糖尿病患者各50例，RT-PCR检测其大网膜脂肪组织中FOXC2 mRNA的表达水平，并分析两组受试者的临床资料与FOXC2 mRNA水平的相关性。
结果与结论：2型糖尿病患者腹部大网膜脂肪组织FOXC2 mRNA表达水平下降；FOXC2 mRNA表达水平在2型糖尿病组与空腹血糖、血压呈负相关，在非2型糖尿病组与空腹血糖呈负相关(P < 0.01)。提示FOXC2与2型糖尿病的关系主要在于可以减轻胰岛素抵抗，改善胰岛素对糖代谢的调节，此外它的表达下降在糖尿病患者血压升高中起到一定的协同作用。

关键词: 叉头框C2(FOXC2), 2型糖尿病, 实时荧光定量PCR, 反转录-PCR, ROC曲线

Abstract:

BACKGROUND: FOXC2 is closely related to the differentiation of adipose cells. It plays an important role in many aspects, such as energy metabolism, resistance of type II diabetes and metabolic syndrome.
OBJECTIVE: To investigate the expression level of FOXC2 mRNA in human ventral adipose tissue of Yunnan Province, and to explore the relations between FOXC2 and type II diabetes.
METHODS: Type II diabetic patients or nondiabetic patients underwent abdominal surgery in Affiliated Hospital of Kunming Medical University were randomly selected as experimental subjects, with 50 cases in each group. The expression level of FOXC2 mRNA in ventral adipose tissue was detected by RT-PCR. The correlation of FOXC2 mRNA expression and subject clinical data was analyzed.
RESULTS AND CONCLUSION: The expression level of FOXC2 mRNA in ventral adipose tissue in type II diabetic patients decreased. The FOXC2 mRNA expression level of diabetic group was inversely related to fasting blood-glucose and blood pressure. The FOXC2 mRNA expression level of nondiabetic group was inversely related to fasting blood-glucose. These findings indicate that FOXC2 may reduce insulin resistance and improve the regulation of insulin on sugar metabolism in type II diabetic patients. In addition, the decrease of FOXC2 expression shows a synergetic effect on blood press raise in diabetic patients.

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