中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (49): 9186-9189.doi: 10.3969/j.issn.1673-8225.2011.49.015

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

嗅鞘细胞和神经干细胞联合移植阿尔茨海默病大鼠脑内的增殖和定向分化

盛宝英，李  洋，姜尧佳，魏春杰，李  莹，任秀敏   

  1. 佳木斯大学附属第一医院神经内科，黑龙江省佳木斯市  154002
  • 收稿日期:2011-06-09 修回日期:2011-08-22 出版日期:2011-12-03 发布日期:2011-12-03
  • 作者简介:盛宝英★，女，1964年生，黑龙江省望奎县人，汉族，1997年佳木斯大学毕业，硕士，主任医师，硕士生导师，主要从事神经干细胞的研究。 sby_n3@sohu.com
  • 基金资助:

    黑龙江省自然科学基金资助项目(D 2008-64)。

Proliferation and directed differentiation of neural stem cells and olfactory ensheathing cells after co-transplantation into the brain of Alzheimer’s disease rats

Sheng Bao-ying, Li Yang, Jiang Yao-jia, Wei Chun-jie, Li Ying, Ren Xiu-min   

  1. Department of Neurology, First Affiliated Hospital of Jiamusi University, Jiamusi  154002, Heilongjiang Province, China
  • Received:2011-06-09 Revised:2011-08-22 Online:2011-12-03 Published:2011-12-03
  • About author:Sheng Bao-ying★, Master, Chief physician, Master’s supervisor, Department of Neurology, First Affiliated Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China SBY-N3@sohu.com
  • Supported by:

    the Natural Science Foundation of Heilongjing Province, No. D2008-64*

PDF

378

被引次数

5

摘要：

背景：阿尔茨海默病大鼠脑内神经元减少，神经干细胞移植后增殖和向神经元分化能力有限。
目的：观察联合移植嗅鞘细胞和神经干细胞在阿尔茨海默病大鼠脑内，嗅鞘细胞对神经干细胞的增殖和向胆碱能神经元分化的影响。
方法：体外培养嗅鞘细胞和神经干细胞，移植前用5-溴脱氧尿嘧啶核苷标记神经干细胞。将生理盐水，神经干细胞和神经干细胞+嗅鞘细胞分别移植入阿尔茨海默病模型大鼠海马。移植7，14，21，28 d后，进行大鼠脑组织切片免疫组织化学染色检测BrdU和ChAT阳性表达。
结果与结论：联合移植组神经干细胞的增殖和分化情况明显优于其他两组，联合移植组BrdU阳性细胞数和ChAT阳性细胞数明显高于神经干细胞移植组和生理盐水组(P < 0.01)。提示嗅鞘细胞能促进移植的神经干细胞在阿尔茨海默病大鼠脑内增殖和向胆碱能神经元的分化。

关键词: 嗅鞘细胞, 神经干细胞, 联合移植, 增殖, 分化

Abstract:

BACKGROUND: The number of neurons is reduced in the brain of Alzheimer’s disease rats, and the proliferation and differentiation ability of transplanted neural stem cells (NSCs) into neurons is limited.
OBJECTIVE: To investigate the effects of olfactory ensheathing cells (OECs) on the proliferation and differentiation of NSCs to cholinergic neurons after co-transplantation into the brain of Alzheimer’s disease rats.
METHODS: OECs and NSCs were separately cultured in vitro, and the NSCs labeled with 5-bromodeoxyuridine (BrdU) before transplantation. Normal saline, NSCs and NSCs+OECs were separately transplanted into the hippocampi of Alzheimer’s rats. At 7, 14, 21 and 28 days after transplantation, the immunohistochemical staining was used to detects the expression of BrdU and choline acetyltransferase (ChAT) in the slice of rat brain tissues to analyze the transplanted cells’ proliferation and differentiation.
RESULTS AND CONCLUSION: The condition of proliferation and differentiation of the NSCs co-transplanted with OECs was the best. The BrdU-positive cells and ChAT-positive cells of the OECs and NSCs co-transplantation group were the most of the three groups (P < 0.01). OECs can promote the proliferation and differentiation of NSCs to cholinergic neurons after co-transplantation in the brain of Alzheimer’s disease rats.

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