中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (48): 8931-8934.doi: 10.3969/j.issn.1673-8225.2011.48.001

• 骨与关节图像与影像 bone and joint imaging •    下一篇

透视引导下穿刺构建兔椎间盘退变模型的影像和病理变化

蒋新华1，张  伶1，陈建宇2，蔡兆熙2，张  娅2，刘珍珍2，谢传淼1，伍尧泮1，吴沛宏1   

  1. 1华南肿瘤学国家重点实验室，中山大学肿瘤防治中心影像与微创介入中心，广东省广州市  510060
    2中山大学孙逸仙纪念医院放射科，广东省广州市510120
  • 收稿日期:2011-08-12 修回日期:2011-09-13 出版日期:2011-11-26 发布日期:2011-11-26
  • 通讯作者: 陈建宇，教授，硕士生导师，中山大学孙逸仙纪念医院放射科，广东省广州市 510120 chenjianyu5562@163.com
  • 作者简介:蒋新华★，男，1983年生，湖南省常德市人，汉族，2010年中山大学毕业，硕士，医师，主要从事肿瘤影像学及分子影像学研究。 170768357@qq.com
  • 基金资助:

    广东省科技计划项目资助（2008B030301318），课题名称：Gd-MSCs移植修复退变椎间盘及MRI活体示踪的实验研究。

Construction of a rabbit intervertebral disc degeneration model induced by puncture under fluoroscopic guidance characterized by imaging and pathology

Jiang Xin-hua1, Zhang Ling1, Chen Jian-yu2, Cai Zhao-xi2, Zhang Ya2, Liu Zhen-zhen2, Xie Chuan-miao1, Wu Yao-pan1, Wu Pei-hong1   

  1. 1State Key Laboratory of Oncology in South China, Department of Medical Imaging and Interventional Radiology, Cancer Center of Sun Yat-sen University, Guangzhou  510060, Guangdong Province, China
    2Radiology Department of Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou  510120, Guangdong Province, China
  • Received:2011-08-12 Revised:2011-09-13 Online:2011-11-26 Published:2011-11-26
  • Contact: Chen Jian-yu, Professor, Master’s supervisor, Radiology Department of Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China chenjianyu5562@163.com
  • About author:Jiang Xin-hua★ Master, Physician, State Key Laboratory of Oncology in South China, Department of Medical Imaging and Interventional Radiology, Cancer Center of Sun Yat-sen University, Guangzhou 510060, Guangdong Province, China 170768357@qq.com
  • Supported by:

    the Science and Technology Research Project of Guangdong Province, No. 2008B030301318*

PDF

374

被引次数

14

摘要：

背景：国内外构建的椎间盘退变动物模型大部分是在手术直视下进行的，手术切开操作对动物的损伤较大。
目的：透视引导下穿刺构建兔椎间盘退变模型，观察椎间盘退变过程中影像学及病理学的变化规律。
方法：透视引导下穿刺新西兰大白兔L2/3、L3/4、L4/5椎间盘，不同时间点进行X射线及MRI扫描与病理学检测，测量椎间盘的T2WI信号强度。
结果与结论：椎间盘穿刺后，随着时间的推移，椎间盘逐渐退变，椎间隙逐渐变窄，椎间盘T2WI信号逐渐减低，椎体边缘骨质增生，髓核、纤维环变性、坏死，最终纤维环完全撕裂。表明透视引导下穿刺后椎间盘逐渐发生退变，病理改变与人类椎间盘退变的病理改变相似，且建模方法具有操作简单，创伤小，成功率高等优点。

关键词: 兔, 椎间盘, 退变, 磁共振, 病理, 影像学

Abstract:

BACKGROUND: Most animal models of disc degeneration are prepared under direct vision during surgery, and surgical incision leads to a larger damage in animals.
OBJECTIVE: To prepare a rabbit intervertebral disc degeneration model by annulus puncture under fluoroscopic guidance, and to investigate the radiology and pathogenesis of intervertebral disc degeneration.
METHODS: Rabbit annulus was punctured under fluoroscopic guidance at L2/3, L3/4, L4/5, the process of disc degeneration was observed in different time intervals after the puncture of the intervertebral disc by X-ray, MRI and histopathology.
RESULTS AND CONCLUSION: After the puncture of the intervertebral disc, there was a progressive degeneration of the disc with decreased signal intensity on T2WI, the disc showed necrosis of nucleus pulposus cells and annulus, and cracks and fissures were evident in the annulus at last. Annulus puncture can induce a progressive, reproducible intervertebral disc degeneration in a rabbit model.

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