中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8879-8882.doi: 10.3969/j.issn.1673-8225.2011.47.035

• 生物材料综述 biomaterial review • 上一篇    下一篇

壳聚糖纳米粒子基因载体的研究现状

李广峰，杨建东   

  1. 扬州大学临床医学院骨科，江苏省扬州市  225001
  • 收稿日期:2011-05-20 修回日期:2011-08-18 出版日期:2011-11-19 发布日期:2011-11-19
  • 通讯作者: 杨建东，博士，副主任医师，扬州大学临床医学院骨科，江苏省扬州市225001 yangjiandong69@sohu.com
  • 作者简介:李广峰★，男，1983年生，山东省聊城人，医师，扬州大学医学院在读硕士，主要从事脊柱脊髓损伤及疾病的研究。 wudi19840315@163.com
  • 基金资助:

    国家自然科学基金项目(81071466)。

Research advances of chitosan nanoparticles as genetic carriers

Li Guang-feng, Yang Jian-dong   

  1. Department of Orthopaedics, Clinical Medical College of Yangzhou University, Yangzhou  225001, Jiangsu Province, China
  • Received:2011-05-20 Revised:2011-08-18 Online:2011-11-19 Published:2011-11-19
  • Contact: Yang Jian-dong, Doctor, Associate chief physician, Department of Orthopaedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu Province, China yangjiandong69@sohu.com
  • About author:Li Guang-feng★, Studying for master’s degree, Physician, Department of Orthopaedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu Province, China wudi19840315@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81071466*

PDF

834

被引次数

11

摘要：

背景：壳聚糖纳米粒子因其独有特性作为基因载体的研究日益增多。
目的：综述了壳聚糖纳米粒子作为基因载体的研究进展，进一步促进基因治疗的效果。
方法：应用计算机检索web of science数据库和中国学术期刊数据库中2000-01/2011-04关于壳聚糖及其衍生物作为基因载体研究的文章，在标题和摘要中以“chitosan，gene”或 “壳聚糖；基因”为检索词进行检索。选择内容与基因载体和壳聚糖相关的文章，初检得到120篇文献，根据纳入标准选择31篇文章进行综述。
结果与结论：壳聚糖基因纳米粒子作为非病毒基因述文章的数量、主要结载体将在基因治疗领域中发挥举足轻重的作用，今后壳聚糖转基因体系的研究将更为深入。如何标记、可视跟踪壳聚糖DNA复合物进入不同细胞的过程，明确其基因转染机制，进一步提高基因转染效率，使其尽快进入基因治疗临床应用是今后研究的主要方向。

关键词: 壳聚糖, 基因治疗, 载体, 制备, 改性

Abstract:

BACKGROUND: In recent years, studies of chitosan as a genetic carrier multiplied for its own characteristics have been increased gradually.
OBJECTIVE: To review the research advances of chitosan nanoparticles as a genetic carrier, and to further improve the effect of gene therapy.
METHODS: Web of Science and CNKI databases were searched (2000-01/2011-04) with the key words of “chitosan, gene” in English and Chinese, respectively. 120 articles related to gene carrier and chitosan were selected firstly, and finally 31 articles were included in result analysis.
RESULTS AND CONCLUSION: Chitosan gene nanoparticle as the non viral gene carrier will play a vital role in this field in the future. Research of chitosan genetically modified system will be more deeply. How to mark, visually track the process of chitosan DNA complex into different cells, make the genetic carrying mechanism clear, improve efficiency, make gene therapy into clinical application as soon as possible will be the main research points in the future.

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