中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8809-8812.doi: 10.3969/j.issn.1673-8225.2011.47.017

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

植入式葡萄糖传感器膜材料壳聚糖与Nafion的组织相容性比较

刘伯基，沙宪政   

  1. 中国医科大学，基础医学院生物医学工程教研室，辽宁省沈阳市 110001
  • 收稿日期:2011-05-19 修回日期:2011-06-26 出版日期:2011-11-19 发布日期:2011-11-19
  • 通讯作者: 沙宪政，教授，中国医科大学，基础医学院生物医学工程教研室，辽宁省沈阳市 110001 xzsha@ mail.cmu.edu.cn
  • 作者简介:刘伯基★，男，1985年生，广东省广州市人，汉族，中国医科大学在读硕士，主要从事传感器生物材料研究。 liuboji85@ 163.com
  • 基金资助:

    辽宁省自然科学基金项目(201102289)。

Histocompatibility of implantable glucose sensor membrane materials: Comparison of chitosan and Nafion

Liu Bo-ji, Sha Xian-zheng   

  1. Department of Biomedical Engineering, China Medical University, Shenyang  110001, Liaoning Province, China
  • Received:2011-05-19 Revised:2011-06-26 Online:2011-11-19 Published:2011-11-19
  • Contact: Sha Xian-zheng, Professor, Department of Biomedical Engineering, China Medical University, Shenyang 110001, Liaoning Province, China xzsha@ mail.cmu.edu.cn
  • About author:Liu Bo-ji★, Studying for master’s degree, in China Medical University, now working in Radiation Therapy Center of Sun·Yat-sen University Cancer Center, Guangzhou, 510060, Guangdong Province, China liuboji85@163.com
  • Supported by:

    the Natural Science Foundation of Liaoning Province, No. 201102289*

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摘要：

背景：壳聚糖是天然高分子多糖，可单独或者与其他材料复合制作敷料、药物、基因载体、生物涂层、组织工程支架、传感器膜材料等。
目的：了解壳聚糖作为植入式葡萄糖传感器膜材料的组织相容性，并与Nafion膜进行对比。
方法：制备壳聚糖膜并对其理化性质进行表征，比较壳聚糖膜皮下植入与肌肉植入、Nafion膜肌肉植入的生物相容性。
结果与结论：壳聚糖膜的厚度、溶胀率、表观密度等理化参数可以通过浓度、铸膜液体积来控制；壳聚糖膜能生物降解，63 d皮下植入的降解率为(17.0±9.9)%，说明壳聚糖的体内降解速度较慢。壳聚糖膜皮下植入引起的炎症反应较肌肉植入重，63 d后形成的纤维包膜比肌肉植入要厚(P < 0.05)；肌肉植入Nafion与壳聚糖膜引起材料周围纤维包膜厚度差异无显著性意义(P > 0.05)，两者均在15 d以后趋于稳定。证明壳聚糖膜能生物降解，与Nafion膜均有较好的组织相容性。

关键词: 壳聚糖, Nafion, 植入式葡萄糖传感器, 组织相容性, 膜材料

Abstract:

BACKGROUND: Chitosan is a natural polysaccharide polymer, and can be applied alone or combined with other materials to prepare dressings, drugs, gene vectors, biological coatings, tissue engineering scaffolds, sensors membranes, etc.
OBJECTIVE: To determine the soft-tissue histocompatibility of chitosan (CS) and Nafion that can be used to optimize the properties of implantable glucose sensors.
METHODS: CS membrane was prepared, characterized, and subcutaneously implanted to investigate the biodegradation property in vivo. Muscularly implanted in mice, the histocompatibility of CS was compared with common sensor material Nafion.
RESULTS AND CONCLUSION: The properties of CS membrane could be controlled through preparation techniques. Compared with muscularly implanted, CS membrane subcutaneously implanted resulted in heavier inflammatory reaction and the average degradation rate was (17.0±9.9)% at 63 days after subcutaneously implanted. The fibrous capsule (FC) thickness was comparable with no statistic difference (P > 0.05) for CS and Nafion, and both became stable after 15 days. Compared with muscularly implanted, FC thickness of CS subcutaneously implanted was thicker. As a result, it is proved that CS and Nafion are both biocompatible to be used in implantable glucose sensor.

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