中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8762-8764.doi: 10.3969/j.issn.1673-8225.2011.47.006

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

整合素α5β1在成骨细胞与生物衍生材料黏附过程中的表达

祁  洁1，张姝江2，刘宗智1   

  1. 1陕西省人民医院骨科，陕西省西安市  710068
    2广州医学院第一附属医院骨科实验室，广东省广州市  510120
  • 收稿日期:2011-07-14 修回日期:2011-09-01 出版日期:2011-11-19 发布日期:2011-11-19
  • 作者简介:祁洁☆，女，1976年生，江苏省南京市人，汉族，2005年四川大学华西医院毕业，博士，副主任医师，主要从事脊柱外科组织工程、再生医学、干细胞方面的研究。 qj_dentist@163.com
  • 基金资助:

    陕西省科学技术研究发展计划项目(2008K14-07)，陕西省自然科学基础研究计划项目(2009JM4017)。

Expression of integrin alpha 5 beta 1 during the adhesion of osteoblasts to bio-derived materials

Qi Jie1, Zhang Shu-jiang2, Liu Zong-zhi1   

  1. 1Department of Orthopeadic Surgery, Shaanxi Provincial People’s Hospital, Xi’an  710068, Shaanxi Province, China
    2Orthopedics Laboratory, First Affiliated Hospital of Gangzhou Medical College, Guangzhou  510120, Guangdong Province, China
  • Received:2011-07-14 Revised:2011-09-01 Online:2011-11-19 Published:2011-11-19
  • About author:Qi Jie☆, Doctor, Associate chief physician, Department of Orthopeadic Surgery, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi Province, China qj_dentist@163.com
  • Supported by:

    the Science and Technology Research and Development Plan of Shaanxi Province, No. 2008K14-07*; the Natural Science Foundation of Shaanxi Province, No. 2009JM4017*

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摘要：

背景：整合素在细胞与材料的黏附过程中发挥重要作用。
目的：了解整合素α5β1在成骨细胞构建组织工程骨和组织工程骨膜过程中的表达，探讨在成骨细胞与生物衍生材料黏附过程中整合素α5β1发挥的作用。
方法：选用人胚骨膜来源成骨细胞为种子细胞，接种生物衍生骨及羊膜支架材料上，制备组织工程骨及骨膜，分别培养2，4，6，8，10 d。单纯培养的成骨细胞作为对照组。
结果与结论：实时定量PCR测定结果，培养早期整合素α5表达呈阴性，整合素β1组织工程骨中的表达略高于组织工程骨膜，培养2，6 d组比较差异有显著性意义。单纯细胞培养的对照组中，整合素β1稳定，各时间段比较差异无显著性意义。提示生物衍生材料有利于成骨细胞黏附，但在黏附过程的初期，成骨细胞可能是通过纤维连接蛋白以外的其他蛋白质完成该过程的。

关键词: 生物衍生材料, 成骨细胞, 整合素&alpha, 5&beta, 1, 黏附, 种子细胞

Abstract:

BACKGROUND: Integrin plays an important role in the cell adhesion process.
OBJECTIVE: To explore the expressions and function of integrinα5β1 during the adhesion of osteoblasts to bio-derived materials.
METHODS: Fetal osteoblasts as seed cells were used to construct tissue engineered bone and periosteum following incubation on the bio-derived materials, which were cultured for 2, 4, 6, 8, 10 days in vitro. Osteoblasts cultured alone served as controls.
RESULTS AND CONCLUSION: Integrinα5 did not express at the early-stage. Integrinβ1 kept expressing in all groups, and expressed higher on bio-derived bone than on tissue-engineered periosteum, especially at the 2nd and 6th days. Integrinβ1 expressed stably in the control group. The bio-derived materials promote osteoblasts adhesion but fibronectin is not essential to the adhesion of osteoblasts.

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