关键词: 异种骨, 生物相容性, 细胞毒性, 急性毒性实验, 热源实验

Abstract:

BACKGROUND: Xenogeneic bone morphology, structure and composition are similar to human bone tissue, and the antigenicity of xenogeneic bone can be decreased after physicochemical treatment, with a natural porous structure. Xenogeneic bone is considered to be an effective way to solve the shortage of the autogenous bone and allograft bone.
OBJECTIVE: To detect the biocompatibility of xenogenic bone materials prepared by three different ways.
METHODS: Three sheep vertebral bone xenograft materials were prepared through physical, chemical and physical combined with chemical methods, and they were made extracted liquid and transplanted into the New Zealand white rabbits in vivo. Acute toxicity test, pyrogen test, intramuscular stimulation test and cytotoxicity test were used to evaluate the biocompatibility to sheep vertebral bone xenograft materials of different treatment groups.
RESULTS AND CONCLUSION: There was no acute toxicity, pyrogen reaction, irritation reaction or cytotoxicity of sheep vertebral bone xenograft materials prepared through physical, physical combined with chemical methods; While there was acute toxicity, pyrogen reaction, mild irritation and cytotoxicity of sheep vertebral bone xenograft materials prepared through chemical methods. The xenogeneic bone materials prepared in the study showed the good biocompatibility through physical, physical combined with chemical methods. However, xenogeneic bone materials prepared through chemical methods, which had poor biocompatibility, could not qualify for the safety standards of the biological materials.