中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8171-8174.doi: 10.3969/j.issn.1673-8225.2011.44.001

• 肝移植 liver transplantation •    下一篇

恒河猴移植肝组织内细胞间黏附分子1与急性排斥反应的关系

冉江华，张升宁，刘  静，李  铸，吴淑媛，李来邦，张熙冰，张鸿青，李  立   

  1. 昆明市第一人民医院暨昆明医学院附属甘美医院肝胆胰一科、云南省器官移植研究所肝移植研究中心，云南省昆明市   650011
  • 收稿日期:2011-07-23 修回日期:2011-08-31 出版日期:2011-10-29 发布日期:2011-10-29
  • 通讯作者: 冉江华，博士，教授，硕士生导师，昆明市第一人民医院暨昆明医学院附属甘美医院肝胆胰一科、云南省器官移植研究所肝移植研究中心，云南省昆明市650011 rjh2u@163.com
  • 作者简介:冉江华☆，男，1965年生，重庆市人，博士，教授，硕士生导师，主要从事器官移植、免疫耐受、肝胆胰外科等方面的研究。
  • 基金资助:

    昆明市科技局重大项目(08S100304)。

Interrelations between acute rejection and intercellular adhesion molecule-1 in liver tissue after liver transplantation in Rhesus monkey

Ran Jiang-hua, Zhang Sheng-ning, Liu Jing, Li Zhu, Wu Shu-yuan, Li Lai-bang, Zhang Xi-bing, Zhang Hong-qing, Li Li   

  1. First Department of Hepato- pancreatobiliary Surgery, Affiliated Ganmei Hospital of Kunming Medical College and First Hospital of Kunming City, Research Center of Liver Transplantation, Yunnan Province Institute of Organ Transplantation, Kunming  650011, Yunnan Province, China
  • Received:2011-07-23 Revised:2011-08-31 Online:2011-10-29 Published:2011-10-29
  • Contact: Ran Jiang-hua, First Department of Hepato- pancreatobiliary Surgery, Affiliated Ganmei Hospital of Kunming Medical College and First Hospital of Kunming City, Research Center of Liver Transplantation, Yunnan Province Institute of Organ Transplantation, Kunming 650011, Yunnan Province, China rjh2u@163.com
  • About author:Ran Jiang-hua☆, Doctor, Professor, Master’s supervisor, First Department of Hepato- pancreatobiliary Surgery, Affiliated Ganmei Hospital of Kunming Medical College and First Hospital of Kunming City, Research Center of Liver Transplantation, Yunnan Province Institute of Organ Transplantation, Kunming 650011, Yunnan Province, China
  • Supported by:

    the Major Program of Bureau of Science and Technology of Kunming City, No. 08S100304*

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摘要：

背景：在急性排斥反应发生时会伴有移植组织中细胞间黏附分子1表达水平的增高。
目的：探讨恒河猴移植肝组织内细胞间黏附分子1与急性排斥反应的关系。
方法：建立恒河猴同种异体肝移植模型，随机分为实验组(移植后不给予抗排斥处理)和对照组(移植中及移植后均给予抗排斥处理)。
结果与结论：实验组移植后24，72 h血清丙氨酸氨基转移酶及血清总胆红素水平明显高于对照组(P < 0.05或< 0.001)。移植后12 h实验组移植肝即表现为轻度急性排斥反应，说明急性排斥反应时肝功能变化滞后于肝组织病理学检查，移植后24，72 h表现为中重度急性排斥反应。移植后6 h实验组肝组织中血管内皮细胞、肝细胞膜、胆管上皮细胞上细胞间黏附分子1的表达呈持续增强，并显著高于对照组(P < 0.05)，随细胞间黏附分子1的表达增强，排斥反应加剧，说明在急性排斥反应早期，肝功能、病理学仅有轻微改变时，细胞间黏附分子1水平即显著升高。提示移植肝组织中细胞间黏附分子1表达的检测对肝移植后急性排斥反应的早期诊断具重要意义。

关键词: 细胞间黏附分子1, 肝移植, 急性排斥反应, 细胞间黏附分子1, 灵长类动物, 恒河猴

Abstract:

BACKGROUND: Intercellular adhesion molecule-1 (ICAM-1) level in transplanted tissue would increase when acute rejection occurs.
OBJECTIVE: To investigate the relationship between ICAM-1 and acute rejection of liver transplantation in Rhesus Monkey.
METHODS: Allogenic liver transplantation models were established in Rhesus monkey and then randomly divided into an experimental group (without anti-rejection management after liver transplantation) and a control group (with anti-rejection management during and after liver transplantation).
RESULTS AND CONCLUSION: At 24 and 72 hours after liver transplantation, serum alanine aminotransferase and serum total bilirubin levels were significantly higher in the experimental group than in the control group (P < 0.05 or P  < 0.001). At 12 hours after liver transplantation, transplanted liver in the experimental group showed mild acute rejection, indicating that during acute rejection, liver function changes occur after pathological examination of liver tissue. At 24 and 72 hours after liver transplantation, moderate and severe acute rejection occurred. At 6 hours after liver transplantation, ICAM-1 expression in the vascular endothelial cells, liver cell membrane, and biliary epithelial cells was significantly increased in the experimental group than in the control group (P  < 0.05). In the experimental group, with ICAM-1 expression increasing, rejection was aggravated, indicating that at the early stage of acute rejection, slight changes in liver function and pathological manifestation were observed, and ICAM-1 level was greatly increased. These findings suggest that detection of ICAM-1 level in the cells of transplanted liver tissue would be of significance for early diagnosis of acute rejection after liver transplantation.

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