中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (42): 7791-7795.doi: 10.3969/j.issn.1673-8225.2011.42.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

负载转基因成肌细胞和骨形态发生蛋白组织工程化骨修复骨缺损

张  力1, 2，王  伟2   

  1. 1辽宁中医药大学，辽宁省沈阳市 110032
    2辽宁医学院第五临床医学院/锦州市中心医院，辽宁省锦州市 121000
  • 收稿日期:2011-01-21 修回日期:2011-04-18 出版日期:2011-10-15 发布日期:2011-10-15
  • 通讯作者: 王伟，博士，教授，博士生导师，辽宁医学院第五临床医学院，锦州市中心医院，辽宁省锦州市 121000 Weiwang_Ly@yahoo.com.cn
  • 作者简介:张力☆，男，1980年生，主治医师，辽宁中医药大学在读博士，主要从事骨缺损、周围神经再生修复及组织工程学方面的研究。 Zhangli2004jz@yahoo.com.cn
  • 基金资助:

    国家自然基金资助项目(30772190)，转分泌性CNTF基因的肌源性干细胞复合支架修复周围神经缺损的实验研究；辽宁省教育厅创新团队项目(2006 123-33)，人去细胞同种异体神经移植物的制备、储存及临床应用研究；辽宁省科技计划项目(2005225003-14)，新型人工生物复合材料修复骨缺损的研究。

Bone defect repair with a new tissue-engineered bone carrying bone morphogenetic protein and transgene myoblasts in rabbits

Zhang Li1, 2, Wang Wei2   

  1. 1Liaoning University of Traditional Chinese Medicine, Shenyang  110032, Liaoning Province, China
    2Fifth Clinical College of Liaoning Medical University (Jinzhou Central Hospital), Jinzhou  121000, Liaoning Province, China
  • Received:2011-01-21 Revised:2011-04-18 Online:2011-10-15 Published:2011-10-15
  • Contact: Wang Wei, Doctor, Professor, Doctoral supervisor, Fifth Clinical College of Liaoning Medical University (Jinzhou Central Hospital), Jinzhou 121000, Liaoning Province, China Weiwang_Ly@ yahoo.com.cn
  • About author:Zhang Li☆, Studying for doctorate, Attending physician, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China; Fifth Clinical College of Liaoning Medical University (Jinzhou Central Hospital), Jinzhou 121000, Liaoning Province, China Zhangli2004jz@ yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30772190*; the Innovation Team Project of Liaoning Educational Bureau, No. 2006 123-33*; Liaoning Provincial Science and Technology Program, No. 2005225003-14*

PDF

363

被引次数

10

摘要：

背景：近年来有报道提出了睫状神经营养因子能够抑制成肌细胞体外成肌分化，可保持成肌细胞去分化状态的理论。两者可通过基因工程技术结合为组织工程化骨的构建提供种子细胞，联合骨形态发生蛋白在一定条件下促进骨缺损的修复。
目的：探讨转染睫状神经营养因子基因成肌细胞与骨形态发生蛋白通过透明质酸钠凝胶载体结合修复兔桡骨节段性缺损的效果。
方法：新西兰大白兔制备左侧桡骨中段造成1.0 cm的节段性骨缺损，缺损处以1.4 cm硅胶管桥接固定，管内植入负载骨形态发生蛋白与转睫状神经营养因子基因成肌细胞的透明质酸凝胶(实验组)、仅复合骨形态发生蛋白的透明质酸凝胶(对照组)或仅植入透明质酸凝胶(空白组)。
结果与结论：动物体内实验表明实验组的X射线阻射密度、大体标本以及病理组织学观察情况均优于对照组和空白组，对照组也优于空白组。说明应用透明质酸钠凝胶复合转基因成肌细胞和骨形态发生蛋白因子作为膜内填充物构建组织工程化骨具有可行性，其应用于修复兔桡骨节段性缺损效果理想。

关键词: 成肌细胞, 基因转染, 睫状神经营养因子, 组织工程, 骨形态发生蛋白

Abstract:

BACKGROUND: In recent years it has been reported that ciliary neurotrophic factor (CNTF) can inhibit the myogenic differentiation of myoblast in vitro to maintain the dedifferentiated status of myoblasts. Myoblasts transfected with CNTF can be used as seed cells for tissue-engineered bone, which combined with bone morphogenetic protein (BMP) under certain conditions can promote the repair of bone defects.
OBJECTIVE: To investigate the effect of transfergene myoblasts in combination with BMP to repair radius defects in rabbits through sodium hyaluronate (HA) gel as a carrier.
METHODS: At aseptic conditions, about 1.0 cm segmental bone defect was produced in the middle of rabbit radius, which was bridged with a 1.4 cm silica tube. Different inserts were implanted into the tube: myoblasts transfected with CNTF and BMP using the HA gel as a carrier (experimental group); BMP only compounded with the HA gel (control group); only HA gel implant group (blank group).
RESULTS AND CONCLUSION: The animal experiments showed that the results of X-ray test, general observation, and pathohistological observation were better in the experimental group than the control and blank groups, and also better in the control group than the blank group. With GTR membrane as a silicone tube, HA gel compound transgenic myoblasts with BMP are feasible to construct a a new type tissue-engineered bone which has good effect on the repair of radius defects.

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