中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7132-7134.doi: 10.3969/j.issn.1673-8225.2011.38.023

• 材料力学及表面改性 material mechanics and surface modification • 上一篇    下一篇

经EX-810处理的食管用修补材料

李  莉1，顾志鹏2，陈  健1，余喜讯2   

  1. 1解放军452医院肿瘤血液中心，四川省成都市  610021
    2四川大学高分子科学与工程学院医用高分子与人工器官系，四川省成都市  610065
  • 收稿日期:2011-03-09 修回日期:2011-05-07 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: 余喜讯，副教授，硕士生导师，四川大学高分子科学与工程学院医用高分子与人工器官系，四川省成都市 610065 yuxixun@163.com
  • 作者简介:李莉★，女，1972年生，四川省宜宾市人，汉族，1993年泸州医学院毕业，硕士，主治医师，主要从事肿瘤血液疾病及生物材料方面的研究。 lzslili@sina.com
  • 基金资助:

    四川省卫生厅科研课题(100194)；四川省科技攻关资助项目(05SG022-022)。

Esophageal repairing acellular biological tissues fixed by EX-810

Li Li1, Gu Zhi-peng2, Chen Jian1, Yu Xi-xun2   

  1. 1Department of Oncology, the 452 Hospital of Chinese PLA, Chengdu  610021, Sichuan Province, China
    2Department of Medical Polymer and Artificial Organs, College of Polymer Science and Engineering, Sichuan University, Chengdu  610065, Sichuan Province, China
  • Received:2011-03-09 Revised:2011-05-07 Online:2011-09-17 Published:2011-09-17
  • Contact: Yu Xi-xun, Associate professor, Master’s supervisor, Department of Medical Polymer and Artificial Organs, College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, Sichuan Province, China yuxixun@163.com
  • About author:Li Li★, Master, Attending physician, Department of Oncology, the 452 Hospital of Chinese PLA, Chengdu 610021, Sichuan Province, China lzslili@sina.com
  • Supported by:

    the Scientific Research Project of Sichuan Health Bureau, No. 100194*; the Science and Technology Tackle Key Program of Sichuan Province, No. 05SG022-022*

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摘要：

背景：天然生物组织来源材料能逐渐被吸收，同时能够诱导组织特异性结构重塑，是理想的食管修补材料。
目的：观察环氧类交联剂EX-810处理的猪食管组织的性能特点。
方法：用4%EX-810处理猪食管组织1~120 h，通过对交联指数的测定观察交联情况；扫描电镜观察组织交联前后的形态学变化；将新鲜的，交联3，7 d的样品进行力学性能测定，观察交联对材料力学性能的影响。
结果与结论：EX-810能有效地交联组织，并能通过去除细胞、组织中的自由氨基等抗原性物质而有效降低生物组织的抗原性；同时还能保持生物组织中胶原的结构形态不受大的破坏；力学性能较处理前也有较大改善，并且还可以改变材料的应力应变曲线形状，使其更加符合食管修补材料的力学要求。提示经EX-810处理的食管修补材料既保持了天然食管材料的结构特征，同时又具有低抗原性、易于保存及更适合的力学性能等优点。

关键词: 猪食管组织, 食管修补材料, 环氧类交联剂(EX-810), 微观结构, 力学性能

Abstract:

BACKGROUND: Natural biological tissue materials can be gradually absorbed, and can induce tissue-specific remodeling, which are ideal esophageal repair materials.
OBJECTIVE: To investigate the characteristics of porcine esophageal tissues fixed with ethylene glycol diglycidyl ether (EX-810).
METHODS: The porcine esophageal tissues were treated with 4% EX-810 for 1 hour to 120 hours, and then they were examined morphologically with the electron microscope. The fixation index determination and the biomechanics test were also performed to study the degree of cross-linking and the effect of cross-linking on the mechanical properties.
RESULTS AND CONCLUSION: The antigenicity of esophageal tissues could be diminished by EX-810 through getting rid of cells in the esophageal tissues or reducing the level of free amino groups in the esophageal tissues. The structural integrity of collagen in esophageal tissues could be preserved after treating with EX-810. In this study, it was also found that the EX-810-fixed porcine esophageal tissues showed better mechanical properties than the native esophageal tissues, and had a changed stress-strain characteristics. It could meet the standard used as the prosthetic materials for bridging esophageal defects. The EX-810-fixed porcine esophageal tissues showed low antigenicity and proper mechanical properties, and the structural integrity of collagen in them could also be preserved.

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