中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7095-7099.doi: 10.3969/j.issn.1673-8225.2011.38.015

• 药物控释材料 drug delivery materials • 上一篇    下一篇

壳聚糖水凝胶微球模板的载肝素层层自组装微胶囊

吴英锋1，郭海利2，李  娟2   

  1. 1首都医科大学宣武医院血管外科，首都医科大学血管外科研究所，北京市  100053
    2中国科学院高能物理研究所纳米材料与纳米生物安全效应实验室，北京市  100049
  • 收稿日期:2011-01-14 修回日期:2011-03-05 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: Li Juan, Ph.D., Assistant researcher, CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China lijuan@ihep.ac.cn
  • 作者简介:吴英锋☆，男，1976年生，河北省衡水市人，汉族，2006年首都医科大学毕业，博士，主治医师，主要从事功能性组织工程血管和体内工程血管研究。
  • 基金资助:

    北京市自然科学基金：在体靶向自组装小口径组织工程动脉的初步研究(课题编号2103049)；北京市自然科学基金：多孔脱细胞小口径组织工程动脉支架及其再细胞化研究(5082007)；北京市科委基金：利用表面仿生分子工程技术制备新型复合抗凝血材料(课题编号Y0905001000091)；北京理工大学研究生创新基金：共价接枝微胶囊新型抗凝血材料的制备与特性(课题编号GA200808)。

Chitosan hydrogel microsphere-templated microcapsules carrying heparin via layer-by-layer self-assembly

Wu Ying-feng1, Guo Hai-li2, Li Juan2   

  1. 1Department of Vascular Surgery, Xuanwu Hospital & Institute of Vascular Surgery, Capital Medical University, Beijing  100053, China
    2CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing  100049, China
  • Received:2011-01-14 Revised:2011-03-05 Online:2011-09-17 Published:2011-09-17
  • Contact: 李娟，博士，助理研究员，主要从事微纳米药物载体和组织工程材料的生物相容性研究。中国科学院高能物理研究所，北京市 100049
  • About author:Wu Ying-feng☆, Doctor, Attending physician, Department of Vascular Surgery, Xuanwu Hospital & Institute of Vascular Surgery, Capital Medical University, Beijing 100053, China wyf799@hotmail.com
  • Supported by:

    the Natural Science Foundation of Beijing, No. 2103049*, 5082007*; Beijing Science and Technology Committee Foundation, No. Y0905001000091*; Graduate Innovation Fund of Beijing Institute of Technology, No. GA200808*

PDF

472

被引次数

1

摘要：

背景：近年来，对抗凝血药物肝素的控释一直存在争议，静电层层自组装是微胶囊载药简单有效的新方法。
目的：制备壳聚糖水凝胶微球模板的载肝素层层自组装微胶囊，从而实现对抗凝血药物肝素的控释。
方法：采用硫酸钠沉淀的方法制备了具有正电荷的壳聚糖微球模板，通过层层自组装的方法装载抗凝血药物肝素。壳聚糖(CS)作为聚阳离子和肝素(Heparin, Hep)作为聚阴离子，在壳聚糖微球的模板上层层自组装形成{CS/Hep}3。{CS/Hep}3包被壳聚糖微球模板的微胶囊通过倒置荧光显微镜、激光共聚焦显微镜和激光粒度分析进行了表征。壳聚糖和肝素的层层自组装过程通过Zeta电位分析进行监测。
结果与结论：{CS/Hep}3包被壳聚糖微球模板的微胶囊平均直径1 μm，包封率和载肝素量分别为83.8%和3.05%。成功制备了壳聚糖水凝胶微球模板的载肝素层层自组装微胶囊，进而实现对抗凝血药物肝素的控释。

关键词: 硫酸钠沉淀法, 壳聚糖, 肝素, 层层自组装, 微胶囊

Abstract:

BACKGROUND: Electrostatic layer-by-layer (LbL) self-assembly is a simple, effective and novel approach to carrying macromolecules into microcapsule carriers.
OBJECTIVE: To prepare chitosan (CS) microcapsules to control the releasing of heparin (Hep) by LbL.
METHODS: CS microspheres were fabricated by a sodium sulfate-based precipitation process and then used as positively charged templates for polyelectrolyte multilayer coatings to carry heparin by the LbL self-assembly technique. The polycation was CS and the polyanion was Hep. The microcapsule was formed via coating {CS/Hep}3 on CS hydrogel template. The microcapsules composed of {CS/ Hep}3 on CS hydrogel templates were confirmed by fluorescence inverted microscopy, confocal laser scanning microscopy and laser particle size analysis. The assembly of polyelectrolyte layers on CS hydrogel microspheres was monitored by Zeta Potential Analyzer.
RESULTS AND CONCLUSION: The resulting microcapsules were about 1 μm in average diameter, and allowed spontaneous loading of heparin through electrostatic interaction, with the encapsulation efficiency and carrying capacity of 83.8% and 3.05%, respectively.

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