中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7090-7094.doi: 10.3969/j.issn.1673-8225.2011.38.014

• 药物控释材料 drug delivery materials • 上一篇    下一篇

胶原-壳聚糖支架与软骨细胞的生长

詹兴旺1，韦曙东1，姜  艳2 ，王文良3，詹兴秀4   

  1. 1武警8750部队医院外科，云南省蒙自市 661100
    2 红河学院理学院化学系，云南省蒙自市 661100
    3天津武警医学院附属医院骨三科，天津市 300162
    4 云南省中医院妇科，云南省昆明市 650021 
  • 收稿日期:2011-01-06 修回日期:2011-02-12 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: 王文良，博士，硕士生导师，天津武警医学院附属医院骨三科，天津市300162 Kingsouthwest2000@yahoo. com.cn
  • 作者简介:詹兴旺★，男，1980年生，汉族，云南省沾益县人，硕士，主要从事软骨组织工程方面研究。 Zhanxingwang1980@126.com
  • 基金资助:

    天津市自然科学基金项目(10JCYBJC10800)；武警医学院科研基金项目(WZK200902)。

Chitosan-collagen scaffold and chondrocytes growth

Zhan Xing-wang1, Wei Shu-dong1, Jiang Yan2, Wang Wen-liang3, Zhan Xing-xiu4   

  1. 1Department of Surgery, 8750 Hospital of Chinese People’s Armed Police Force, Mengzhi  661100, Yunnan Province, China
    2Department of Chemistry, College of Science, Honghe University, Mengzhi  661100, Yunnan Province, China
    3Third Department of Orthopedics, Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin  300162, China
    4Department of Gynecology, Yunnan Hospital of Traditional Chinese Medicine, Kunming  650021, Yunnan Province, China
  • Received:2011-01-06 Revised:2011-02-12 Online:2011-09-17 Published:2011-09-17
  • Contact: Wang Wen-liang, Doctor, Master’s supervisor, Third Department of Orthopedics, Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China Kingsouthwest2000@yahoo.com.cn
  • About author:Zhan Xing-wang★, Master, Department of Surgery, 8750 Hospital of Chinese People’s Armed Police Force, Mengzhi 661100, Yunnan Province, China zhanxingwang1980@126.com
  • Supported by:

    the Natural Science Foundation of Tianjin, No.10JCYBJC10800*; the Science and Technology Foundation of Medical College of Chinese People’s Armed Police Force, No. WZK200902*

PDF

721

被引次数

29

摘要：

背景：目前软骨支架材料的种类比较多，但还没有一种材料能完全符合软骨修复的要求。
目的：观察在混合材料胶原-壳聚糖支架中软骨细胞的生长情况。
方法：采用冷冻干燥法将质量分数为2%胶原与3%壳聚糖混合制备胶原-壳聚糖多孔支架。将分离培养的第2代兔软骨细胞接种到胶原-壳聚糖支架上，对照组将软骨细胞接种到无支架的培养板中。观察支架的孔隙率、吸水性及内部形态结构，MTT法检测软骨细胞在支架上的增殖情况，组织切片苏木精-伊红染色，扫描电镜观察细胞在支架的生长、贴附情况，RT-PCR检测细胞支架复合物蛋白聚糖和Ⅱ型胶原mRNA表达情况。
结果与结论：胶原-壳聚糖支架的吸水性为(80.0±0.55)%，孔隙率为(88.5±1.5)%，孔径为100~150 μm，复合细胞培养2周后，细胞增殖活力高，软骨细胞分泌的蛋白聚糖和Ⅱ型胶原mRNA表达明显高于对照组。说明质量分数为2%胶原与3%壳聚糖的混合支架适合软骨细胞生长和快速增殖，是一种良好的修复和重建软骨载体。

关键词: 胶原, 支架, 壳聚糖, 软骨细胞, 生物材料与组织工程

Abstract:

BACKGROUND: At present, the materials of cartilage framework are various, but not one kind of material can fully comply with requirements of cartilage repair.
OBJECTIVE: To observe the growth of cartilage cells in mixed chitosan-collagen scaffold.
METHODS: The porous chitosan-collagen scaffold was fabricated by the freeze-drying technique using 2% collagen and 3% chitosan. The chondrocytes at passage 2 were planted in chitosan-collagen scaffolds (experimental group) or cell panels (control group). The porosity, water absorption and internal structure of the scaffold were observed. The cell attachment capacity and proliferation of chondrocytes was valuated by MTT and hematoxylin-eosin staining. Expressions of type Ⅱ collagen and proteoglycan mRNA were valuated by RT-PCR.
RESULTS AND CONCLUSION: The water absorption of chitosan-collagen scaffold was (80.0±0.55)%, porosity was (88.5±1.5)%, and the pore size was 100-150 μm. The scaffold facilitated the penetration and attachment of chondrocytes. After two weeks, RT-PCR showed that collagen and proteoglycan mRNA were expressed in the experimental group, but only a little in the control group. These results showed that the chitosan-collagen scaffold (2% collagen and 3% chitosan) can provide an appropriate environment for the generation of chondrocytes and have a potential application in the cartilage tissue engineering scaffold field. It is one kind of good carrier for restoration and reconstruction of cartilage.

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