中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6919-6922.doi: 10.3969/j.issn.1673-8225.2011.37.019

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

间歇缺氧及睡眠剥夺大鼠血管内皮细胞相关指标的变化

袁春华1，徐劲松2，郭洋琴1，宋宁燕2，夏国际2   

  1. 1江西科技师范学院体育学院，江西省南昌市  330013
    2解放军第94医院呼吸内科，江西省南昌市  330002
  • 收稿日期:2011-03-19 修回日期:2011-06-08 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 徐劲松，硕士，副主任医师，解放军第94医院呼吸内科，江西省南昌市 330002 xujinsong@126.com
  • 作者简介:袁春华☆，女，1971年生，江西省峡江县人，汉族，2007年南方医科大学毕业，博士，副教授，主要从事生理学基础的研究。 yuanchunhua@ 126.com
  • 基金资助:

    江西省科技厅社会发展项目(赣科发计字[2008]230号)，课题名称：运动对OSAS致高血压大鼠的影响及机制研究；南昌市科技局重点攻关项目(YLWS-SHFZ-469)，课题名称：K+离子通道在睡眠呼吸暂停综合征诱发高血压中的作用研究；南京军区医学科学技术研究“十一五”计划资助项目(06MA79)，课题名称：平滑肌离子通道机制在睡眠呼吸暂停综合征诱发高血压中的作用。

Changes in vascular endothelial cells-related indices in rats with intermittent hypoxia and sleep deprivation

Yuan Chun-hua1, Xu Jin-song2, Guo Yang-qin1, Song Ning-yan2, Xia Guo-ji2   

  1. 1School of Physical Education, Jiangxi Science & Technology Normal University, Nanchang   330013, Jiangxi Province, China
    2Department of Respiratory Diseases, the 94 Hospital of Chinese PLA, Nanchang   330002, Jiangxi Province, China
  • Received:2011-03-19 Revised:2011-06-08 Online:2011-09-10 Published:2011-09-10
  • Contact: Xu Jin-song, Master, Associate chief physician, Department of Respiratory Diseases, the 94 Hospital of Chinese PLA, Nanchang 330002, Jiangxi Province, China xujinsong@126.com
  • About author:Yuan Chun-hua☆, Doctor, Associate professor, School of Physical Education, Jiangxi Science & Technology Normal University, Nanchang 330013, Jiangxi Province, China yuanchunhua@126. com
  • Supported by:

    Social Development Program of Science and Technology Department of Jiangxi Province, No. gankefajizi[2008]230*; High-Tech Research and Development Program of Department of Science and Technology of Nanchang, No. YLWS-SHFZ-469*; Eleventh Five-year Development Program of Nanjing Military Area Command Medical Science and Technology Research, No. 06MA79*

PDF

371

被引次数

10

摘要：

背景：血管内皮功能损坏是睡眠呼吸暂停的病理基础。
目的：观察间歇缺氧、睡眠剥夺对SD大鼠有创动脉收缩压及血浆一氧化氮、内皮素、降钙素基因相关肽水平的影响。
方法：将3月龄雄性SD大鼠16只随机等分为2组，模型组大鼠每天置入睡眠剥夺合并间歇性缺氧条件10 h (22：00-08：00)，单纯睡眠剥夺条件12 h(08：00-20：00)，剩余时间置大鼠笼饲养。对照组无睡眠剥夺、无缺氧条件饲养。
结果与结论：造模8周后，与对照组比较，模型组大鼠有创动脉压明显升高(P < 0.01)，血浆一氧化氮、降钙素基因相关肽水平显著降低(P < 0.01)，血浆内皮素水平显著升高(P < 0.01)。说明间歇性缺氧、睡眠剥夺可以引起SD大鼠血压增高，血管内皮功能受损。

关键词: 血管内皮功能, 大鼠, 一氧化氮, 内皮素, 降钙素基因相关肽

Abstract:

BACKGROUND: Sleep apnea syndrome has been considered be a significant independent factor that caused outbreaks of hypertension and coronary heart disease. Vascular endothelial dysfunction may be one of the most important mechanisms for the manifestation of these diseases.
OBJECTIVE: To investigate the effects of intermittent hypoxia and sleep deprivation on invasive arterial blood pressure, plasma nitric oxide, endothelin and calcitonin gene related protein levels in rats.
METHODS: Sixteen 3-month-old male Sprague-Dawley rats were randomly and evenly divided into two groups: the control group and the intermittent hypoxia and sleep deprivation group. Rats in the intermittent hypoxia and sleep deprivation group were placed in an environment of intermittent hypoxia and sleep deprivation for 10 hours (22:00-08:00) and in an environment of simple sleep deprivation for 12 hours (08:00-20:00). During the remaining time, the rats were raised in cages. The control group rats were raised without sleep deprivation and intermittent hypoxia.
RESULTS AND CONCLUSION: After 8 weeks of model establishment, compared with the control group, invasive arterial blood pressure was significantly higher (P < 0.01), plasma nitric oxide and calcitonin gene related protein levels were significantly decreased (P< 0.01), and plasma endothelin level was significantly increased (P < 0.01) in the intermittent hypoxia and sleep deprivation group. These results showed that intermittent hypoxia exposure and sleep deprivation can cause increased blood pressure and vascular endothelial dysfunction in Sprague-Dawley rats.

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