中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5258-5261.doi: 10.3969/j.issn.1673-8225.2011.28.032

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

皮肤扩张器的改造及其在构建自体复合皮中的应用

何丽霞，艾剑雄，杨任欢，牙祖蒙   

  1. 重庆医科大学附属第二医院整形美容外科，重庆市 400010
  • 收稿日期:2011-03-31 修回日期:2011-05-21 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 牙祖蒙，教授，主任医师，重庆医科大学附属第二医院整形美容外科，重庆市 400010 yazumeng@163.com
  • 作者简介:何丽霞★，女，1978年生，硕士，主要从事体表组织器官缺损的形态和功能修复方面的研究。 helixia1306@ sina.com
  • 基金资助:

    重庆市自然科学基金计划项目(CSTC,2007BB5315)

Reformation of soft tissue expander and its application in construction of autologous composite skin

He Li-xia, Ai Jian-xiong, Yang Ren-huan, Ya Zu-meng   

  1. Department of Plastic Surgery, Second Affiliated Hospital of Chongqing Medical University，Chongqing 400010, China
  • Received:2011-03-31 Revised:2011-05-21 Online:2011-07-09 Published:2011-07-09
  • Contact: Ya Zu-meng, Professor, Chief physician, Department of Plastic Surgery, Second Affiliated Hospital of Chongqing Medical University，Chongqing 400010, China yazumeng@163.com
  • About author:He Li-xia★, Master, Department of Plastic Surgery, Second Affiliated Hospital of Chongqing Medical University，Chongqing 400010, China helixia1306@sina. com
  • Supported by:

    the Natural Science Foundation of Chongqing, No. CSTC,2007BB5315*

PDF

445

被引次数

2

摘要：

背景：置入正常皮肤软组织下的皮肤软组织扩张器，通过定期向扩张囊内注入生理盐水使其不断扩张，可获得“额外”皮肤软组织。
目的：对传统皮肤扩张器进行改造并应用于构建自体复合皮。
方法：将传统单注射壶皮肤扩张器改造成双注射壶，原有的注射壶通向扩张囊内，添置的注射壶通向囊外，扩张囊容量10 mL。将改造后的扩张器埋植于10只新西兰大耳白兔背部皮下，扩张器埋置2周后，经注射壶注入原代培养的自体表皮细胞悬液，即种植于扩张囊和纤维包囊的腔隙内。
结果与结论：新西兰大耳白兔完全存活，扩张器未出现破损渗漏，伤口均愈合，无排异反应。注入表皮细胞种植1周后，纤维包囊表面可见较多的上皮细胞岛，但未形成完整的细胞层；2周后的纤维包囊表面可见完整的类似于假复层鳞状上皮的细胞层，两者结合紧密。提示经改造的新型皮肤扩张器用于体内构建自体复合皮是可行的，发挥着关键性的作用。

关键词: 皮肤扩张器, 改良, 纤维包囊, 自体复合皮, 皮肤组织工程

Abstract:

BACKGROUND: Periodical injection of normal saline into the capsular space of soft tissue expander implanted beneath the normal skin soft tissue can expand the capsular space to acquire supplementary skin soft tissue.
OBJECTIVE: To reform the traditional soft tissue expander and then apply it to construct autologous composite skin.
METHODS: One injection pot that lead to the extracapsular space was added to the traditional soft tissue expander. The modified expanders were implanted on the back of 10 New Zealand white rabbits subcutaneously. Two weeks later, the cultured autologous epidermal cell suspension was injected into the space between the fibrous cyst and expander.
RESULTS AND CONCLUSION: All the animals survived with the wounds well healing until the study finished. Expander did not exhibit breakage or leakage. Gross examination showed that 1 week later, epithelial islands without complete epithelial layer appeared on the surface of fibrous cyst; 2 weeks later, complete epithelial layer similar to stratified squamous epithelium was observed on the surface of fibrous cyst. These findings suggest that it is feasible to construct autologous composite skin in vivo using the reformed soft tissue expander.

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