中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (21): 3829-3833.doi: 10.3969/j.issn.1673-8225.2011.21.007

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

脱细胞猪小肠黏膜下层支架复合软骨细胞构建组织工程软骨

吴  迪1，谢  瑜2，王少云1，朱晓松1，李  青1，董  坚3，李世和1   

  1. 昆明医学院第一附属医院，1骨科；2血液科；3生物治疗中心，云南省昆明市  650032
  • 收稿日期:2010-11-12 修回日期:2011-03-25 出版日期:2011-05-21 发布日期:2011-05-21
  • 通讯作者: 谢瑜，硕士，主治医师，昆明医学院第一附属医院血液科，云南省昆明市 650032
  • 作者简介:吴迪☆，男，1974年生，云南省昆明市人，汉族，2008年昆明医学院毕业，博士，副主任医师，主要从事创伤骨科。 xywudi@sohu.com
  • 基金资助:

    云南省社会发展计划(面上项目)(2008ZC117M)。

Tissue engineered cartilage constructed by acellular porcine small intestinal submucosa scaffold combined with autologous chondrocytes

Wu Di1, Xie Yu2, Wang Shao-yun1, Zhu Xiao-song1, Li Qing1, Dong Jian3, Li Shi-he1   

  1. 1Department of Orthopedics, 2Department of Hematology, 3Centre of Biotherapy, First Affiliated Hospital, Kunming Medical University, Kunming  650032, Yunnan Province, China
  • Received:2010-11-12 Revised:2011-03-25 Online:2011-05-21 Published:2011-05-21
  • Contact: Xie Yu, Master, Attending physician, Department of Hematology, First Affiliated Hospital, Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Wu Di☆, Doctor, Associate chief physician, Department of Orthopedics, First Affiliated Hospital, Kunming Medical University, Kunming 650032, Yunnan Province, China xywudi@sohu.com
  • Supported by:

    Social Development Plan of Yunnan Province, No. 2008ZC117M*

PDF

429

被引次数

1

摘要：

背景：关节软骨损伤后无论是否施加干预，都难以达到满意的修复效果。
目的：观察以猪自体软骨细胞为种子细胞复合脱细胞猪小肠黏膜下层构建组织工程软骨的可行性。
方法：将培养至第3代的猪膝关节软骨细胞接种于小肠黏膜下层膜上，复合培养48 h，构建细胞-载体复合物，光学显微镜、扫描电镜观察软骨细胞在小肠黏膜下层膜上的生长情况。
结果与结论：苏木精-伊红染色见细胞在小肠黏膜下层基质层表面呈单层或复层生长；免疫组织化学染色结果显示软骨细胞与小肠黏膜下层表面之间形成一条连续阳性表达条带；扫描电镜见软骨细胞在支架孔隙内贴壁良好生长。

关键词: 小肠黏膜下层, 关节软骨, 自体, 组织工程软骨, 猪

Abstract:

BACKGROUND: It is difficult to achieve a satisfactory repair effect on articular cartilage injury with or without intervention.
OBJECTIVE: To study the feasibility of constructing tissue engineered cartilage using cultivated the autologous chondrocytes combined acellular porcine small intestinal submucosa (SIS) scaffold.
METHODS: The third-generation chondrocytes from porcine knee joint were incubated on porcine SIS and cultured for 48 hours to construct a chondrocyte-scaffold complex. The growth of chondrocytes on the scaffold was observed under light microscope and scanning electron microscope.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining results showed that the chondrocytes presented monolayer or multilayer growth on the surface of the SIS scaffold. Immunohistochemistry staining results showed that there was a successive positive expression strap between the chondrocytes and SIS. Under the scanning electron microscope, the cells adhered to the SIS scaffold grew well.

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