中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (21): 3801-3804.doi: 10.3969/j.issn.1673-8225.2011.21.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

骨髓间充质干细胞与小肠黏膜下层复合构建组织工程骨膜

王军胜1，王栓科2，陈兴礼1，胡小吾1   

  1. 1淮安市第二人民医院骨科，江苏省淮安市  223002
    2 兰州大学第二附属医院骨科，甘肃省兰州市  730000
  • 收稿日期:2010-10-25 修回日期:2010-12-16 出版日期:2011-05-21 发布日期:2011-05-21
  • 作者简介:王军胜★，男，1974年生，甘肃省通渭县人，汉族，2007年兰州大学毕业，硕士，主治医师，主要从事骨组织工程研究。 nick121@sina.com

Tissue engineered periosteum fabricated using bone marrow mesenchymal stem cells and small intestinal submucosa

Wang Jun-sheng1, Wang Shuan-ke2, Chen Xing-li1, Hu Xiao-wu1   

  1. 1Department of Orthopaedics, Huaian Second People’s Hospital, Huaian 223002, Jiangsu Province, China
    2Department of Orthopaedics, Second Affiliated Hospital of Lanzhou University, Lanzhou   730000, Gansu Province, China
  • Received:2010-10-25 Revised:2010-12-16 Online:2011-05-21 Published:2011-05-21
  • About author:Wang Jun-sheng★, Master, Attending physician, Department of Orthopaedics, Huaian Second People’s Hospital, Huaian 223002, Jiangsu Province, China nick121@sina.com

PDF

333

被引次数

5

摘要：

背景：用骨膜修复骨缺损，已被大量实验所证实。但其来源有限，限制了临床大量应用。因此，应用组织工程学的原理和方法构建人工骨膜，值得进一步探索和研究。
目的：将经成骨诱导的兔骨髓来源的间充质干细胞与猪小肠黏膜下层复合构建组织工程骨膜。
方法：取健康新西兰幼兔骨髓，密度梯度离心法分离骨髓间充质干细胞并进行体外扩增培养、成骨诱导分化及鉴定。将诱导分化的骨髓间充质干细胞与猪小肠黏膜下层复合构建组织工程骨膜。观察细胞在生物材料上附着、生长、增殖及细胞分泌细胞外基质情况。
结果与结论：经成骨诱导的兔骨髓间充质干细胞在猪小肠黏膜下层上黏附、增殖速度加快，并能长入材料的孔隙内，分泌大量的细胞外基质成分，骨膜厚度随复合培养时间的延长而增加，类似生物骨膜。说明将经成骨诱导的骨髓间充质干细胞与小肠黏膜下层复合可以构建具有生理功能的组织工程骨膜。

关键词: 小肠黏膜下层, 骨髓间充质干细胞, 兔, 组织工程骨膜, 骨组织工程

Abstract:

BACKGROUND: Periosteum has been confirmed to repair bone defects in large number of experiments. But its source is limited, thus restricting clinical applications. Therefore, artificial periosteum constructed using tissue engineering principles and methods is worthy of further exploration and research.
OBJECTIVE: To explore the practicability of tissue engineered periosteum fabrication by small intestinal submucosa as a tissue scaffold with rabbit bone marrow mesenchymal stem cells (BMSC).
METHODS: Bone marrow was obtained from healthy New Zealand rabbits, by using density gradient centrifugation BMSCs were isolated and were then cultured in vitro. Following induced differentiation and identification, the BMSCs were compounded with porcine small intestinal submucosa to fabricate tissue engineered periosteum. The adhesion, growth, proliferation of BMSCs on the materials, as well as extracellular matrix secretion were observed.
RESULTS AND CONCLUSION: The BMSCs by osteogenic induction could quickly adhere and proliferate on the surface of porcine small intestinal submucosa and got into the holes. Meantime, BMSCs secreted a great deal of extracellular matrices. BMSCs grew well there in layers, and the periosteum became thicker as time passed, acting like a biological periosteum. Combined culture of porcine small intestinal submucosa and BMSCs can construct a tissue engineering periosteum with physiological function.

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