中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2079-2082.doi: 10.3969/j.issn.1673-8225.2011.11.044

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

电针干预膝骨关节炎过程中转化生长因子β的调控作用

吴耀持，孙毅君，周景辉，李石胜   

  1. 上海交通大学附属第六人民医院针推伤科，上海市 200233
  • 收稿日期:2010-08-14 修回日期:2010-09-13 出版日期:2011-03-12 发布日期:2011-03-12
  • 作者简介:吴耀持★，男，1961年生，浙江省湖州市人，汉族，1984年上海中医药大学毕业，硕士，主任医师，教授，博士生导师，主要从事中西医结合治疗腰腿痛的临床与基础研究。
  • 基金资助:

    上海市科学技术委员会科技发展基金项目(08DZ1973200)，透刺温针灸治疗膝骨关节炎临床疗效验证。

Regulatory effects of transforming growth factor beta in electro-acupuncture treatment of knee osteoarthris

Wu Yao-chi, Sun Yi-jun, Zhou Jing-hui, Li Shi-sheng   

  1. Department of Acupuncture and Massage, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai   200233, China
  • Received:2010-08-14 Revised:2010-09-13 Online:2011-03-12 Published:2011-03-12
  • About author:Wu Yao-chi★, Master, Professor, Chief physician, Doctoral supervisor, Department of Acupuncture and Massage, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China wuyaochi@online.sh.cn
  • Supported by:

    Science and Technology Development Foundation of Shanghai Science and Technology Committee, No. 08DZ1973200*

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摘要：

背景: 电针治疗膝骨关节炎具有良好的中枢和外周镇痛作用。研究显示转化生长因子β在膝骨关节炎发生中具有重要作用。
目的：从转化生长因子β调控角度，认识电针治疗膝骨关节炎的可能作用机制。
方法：健康3月龄雄性SD大鼠，随机分为正常组、模型组、电针组和药物组。通过结扎大鼠股静脉并强迫运动的方法，建立膝骨关节炎动物模型。造模1个月后，电针组刺激内膝眼(EX-LE4)和犊鼻(ST 35)穴位，深0.1寸，脉冲2 Hz刺激20 min，1次/d，药物组为关节腔内注射药物透明质酸钠，0.1 mL/次，1次/周。治疗2周后，采集各组动物膝关节滑膜组织，观察转化生长因子β1、转化生长因子β1受体Ⅰ、转化生长因子β受体Ⅱ的表达。
结果与结论：膝关节骨关节炎动物膝关节滑膜中转化生长因子β1水平增加(P < 0.05)，经电针或透明质酸钠治疗后，膝关节滑膜中转化生长因子β1水平降低(P < 0.05)，且转化生长因子β1受体Ⅰ，Ⅱ含量出现显著降低(P < 0.05)。提示电针治疗膝骨关节炎是通过下调转化生长因子β1的含量来改善骨关节炎症状的，受体含量的减少有助于膝骨关节炎的恢复。

关键词: 转化生长因子&beta, 荧光定量PCR, 膝骨关节炎, 电针, 组织构建, 组织工程

Abstract:

BACKGROUND: Electro-acupuncture therapy shows good central and peripheral analgesic effects. Several studies have shown that transforming growth factor β (TGF-β) plays an important role in the pathogenesis of knee osteoarthritis.
OBJECTIVE: To investigate the mechanism underlying electro-acupuncture treatment of knee osteoarthritis.
METHODS: A total of 80 healthy male 3-month-old Sprague Dawley rats were randomly and evenly divided into normal, model, electro-acupuncture and drug groups. Rat models of knee osteoarthris were estbalished by ligating the femoral veins and forcing rats to do activies. At 1 month after knee osteoarthris induction, the electro-acupuncture group rats received electro-acupuncture therapy at two acupoints Neixiyan (EX-LE4) and Dubi (ST 35) with a depth of 0.1 cun (pulse 2 Hz, 20 minutes, once a day). The drug group rats were intraarticularly administerd sodium hyaluronate (0.1 mL/administration, once a week). After 2-week treatment, synovial tissue of the knee joint was harvested to determine the exprssion of TGF-β1, TGF-β1 receptor Ⅰand TGF-β1 receptor Ⅱ.
RESULTS AND CONCLUSION: TGF-β1 expression in synovial tissue of the knee joint was significantly increased after knee osteoarthris (P < 0.05), but after electro-accupuncture therepy or sodium hyaluronate treatment, TGF-β1 expression was significantly decreased (P < 0.05), moreover, TGF-β1 receptor Ⅰ, Ⅱ expression was signficantly decreased (P < 0.05). These findings suggest that electro-acupuncture for treatment of knee osteoartheis improves the symptoms of osteoarthris by downregulating TGF-β1 expression, and reduction in TGF-β1 receptor Ⅰ, Ⅱ expression promotes the recovery of knee osteoarthris.

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