中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2074-2078.doi: 10.3969/j.issn.1673-8225.2011.11.043

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

创伤性深静脉血栓模型大鼠组织蛋白酶B，C基因对血管内皮细胞的作用

姚黎清1，宁  亚1，赵学凌1， 章玉冰1，李宏昆1，李  文2   

  1. 1昆明医学院第一附属医院骨科，云南省昆明市  650032
    2云南省人民医院心内科，云南省昆明市  650032
  • 收稿日期:2010-09-03 修回日期:2010-10-22 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 赵学凌，博士，主任医师，博士生导师，昆明医学院第一附属医院骨科，云南省昆明市 650032
  • 作者简介:姚黎清☆，女，1969年生，云南省丽江市人，昆明医学院在读博士，副主任医师，硕士研究生导师，主要从事临床康复基础与临床研究。

Effects of cathepsin B and cathepsin C gene on vascular endothelial cells in a rat model of traumatic deep venous thrombosis

Yao Li-qing1, Ning Ya1, Zhao Xue-ling1, Zhang Yu-bing1, Li Hong-kun1, Li Wen2   

  1. 1Department of Orthopedics, First Affiliated Hospital, Kunming Medical College, Kunming   650032, Yunnan Province, China
    2Department of Cardiology, Yunnan People’s Hospital, Kunming  650032, Yunan Province, China
  • Received:2010-09-03 Revised:2010-10-22 Online:2011-03-12 Published:2011-03-12
  • Contact: Zhao Xue-ling, Doctor, Chief physician, Doctoral supervisor, Department of Orthopedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China zhaoxuelin@vip. km169.net
  • About author:Yao Li-qing☆, Studying for doctorate, Associate chief physician, Master’s supervisor, Department of Orthopedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China Yaoliqing98731@ yahoo.com.cn

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摘要：

背景：骨科手术后易出现深静脉血栓形成，但目前临床上对此尚缺乏有效预测诊断手段，组织蛋白酶可能是血栓形成的有效生物标记物。
目的：观察大鼠深静脉血栓形成前后组织蛋白酶B和组织蛋白酶 C在血细胞中的表达变化情况，探讨二者作为深静脉血栓形成早期诊断候选分子标志物的可行性。
方法：将100只SD大鼠随机分为正常对照组和模型组，模型组采用血管钳夹股静脉+双后肢固定制动的方式建立大鼠创伤性深静脉血栓模型，根据观察时间点和血栓形成情况分为血栓形成前组、血栓形成高峰期组和血栓不形成组，提取各组血液RNA并反转录为cDNA，应用实时荧光定量PCR检测组织蛋白酶 B和组织蛋白酶 C在血细胞中的表达变化情况。
结果与结论：血栓形成高峰期组大鼠血细胞中组织蛋白酶B，C 表达明显，血栓形成前组和血栓不形成组大鼠血细胞中组织蛋白酶B，C表达于正常对照组大鼠为无明显差异。提示组织蛋白酶B和组织蛋白酶C与深静脉血栓形成密切相关，可作为深静脉血栓形成早期诊断的候选分子标志物。

关键词: 深静脉血栓形成, 组织蛋白酶, 早期诊断, 分子标志物, 大鼠

Abstract:

BACKGROUND: Deep venous thrombosis (DVT) always occurs after orthopedic surgery. At present, clinical diagnosis of DVT has been lack of an effective measuring means for a long time. Cathepsin may be an effective biological marker of DVT.
OBJECTIVE: To study the expression change of cathepsin B and cathepsin C in the rat blood cells before and after DVT and to investigate the feasibility of cathepsin B and cathepsin C as candidate molecular markers for early diagnosis of DVT.
METHODS: Totally 100 Sprague Dawley rats were randomly divided into normal control group (n=10) and model group (n=90). Rat traumatic deep vein thrombosis models were established by clamping the femoral vein and fixing the bilateral hind limbs. According to observation time points and the different situations of thrombosis, rat models were assigned to three subgroups: pre-thrombosis, intra-thrombosis, and non-thrombosis. Blood RNA of each group was extracted and reverse transcribed into cDNA. The expression of cathepsin B and cathepsin C in blood cells was detected using real-time fluorescence quantitative PCR. 
RESULTS AND CONCLUSUON: Expression of cathepsin B and cathepsin C in the blood cells was obviously expressed in the intra-thrombosis subgroup. There was no significant difference in cathepsin B and cathepsin C expression between pre-thrombosis, non-thrombosis groups and normal control group. These findings suggest that cathepsin B and cathepsin C are closely related to DVP and they can be used as the candidate molecular markers for early diagnosis of DVT.

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