中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2051-2054.doi: 10.3969/j.issn.1673-8225.2011.11.038

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

应激动物模型构建方法及效果评价的研究进展

娜  琴1，程  飚2，陈阿梅1   

  1. 1内蒙古医学院组织胚胎学教研室，内蒙古自治区呼和浩特市 010059
    2解放军广州军区广州总医院整形外科，广东省广州市 510010
  • 收稿日期:2010-09-10 修回日期:2010-10-25 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 程飚，博士，教授，解放军广州军区广州总医院整形外科，广东省广州市 510010 chengbiaocheng@163.com 陈阿梅，硕士，教授，内蒙古医学院组织胚胎学教研室，内蒙古自治区呼和浩特市 010059 chenam01@163.com
  • 作者简介:娜琴★，女，1984年生，内蒙古自治区呼和浩特市人，蒙古族，内蒙古医学院在读硕士，主要从事应激与创面愈合研究。 752170727@qq.com
  • 基金资助:

    全军医学科学技术研究“十一五”计划面上项目(08G027)。

Progress addressing animal stress model construction and effect evaluation

Na Qin1, Cheng Biao2, Chen A-mei1   

  1. 1Department of Histology and Embryology, Inner Monglia Medical College, Huhhot  010059, Inner Monglia Autonomous Region, China
    2Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou  510010, Guangdong Province, China
  • Received:2010-09-10 Revised:2010-10-25 Online:2011-03-12 Published:2011-03-12
  • Contact: Cheng Biao, Doctor, Professor, Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China chengbiaocheng@ 163.com Chen A-mei, Master, Professor, Department of Histology and Embryology, Inner Monglia Medical College, Huhhot 010059, Inner Monglia Autonomous Region, China
  • About author:Na Qin★, Studying for master’s degree, Department of Histology and Embryology, Inner Monglia Medical College, Huhhot 010059, Inner Monglia Autonomous Region, China 752170727@qq.com
  • Supported by:

    the General Program of Military Medical Sciences Plan during the Eleventh Five-Year plan, No. 08G027*

PDF

712

被引次数

20

摘要：

背景：应激与疾病的关系越来越被人们所关注，应激动物模型的建立是应激研究的基础。但在应激动物建模方面尚无统一的标准。
目的：就各类应激动物模型的建立方法及建立模型后应激效果的评价进行综述，并分析模型建立及评价中存在的问题。
方法：应用计算机检索PubMed数据库(2000/2010)，以“stress，animal model，murine，evaluate”为检索词；检索重庆维普数据库(2000/2010)，以“应激，动物模型”为检索词。文章所述内容需与应激动物模型的建立、应用、评价，及应激与疾病的关系等方面研究密切相关，排除重复性研究。
结果与结论：共收集417篇关于应激动物模型的文献，中文115篇，英文302篇。阅读标题和摘要进行初筛，排除发表时间较早、重复及类似的研究，纳入30篇符合标准的文献。目前用于研究的应激动物模型种类繁多，但造模方法还没有统一标准，对应激的程度也没有可量化的指标，在应激的评价方面存在缺陷。随着神经科学、分子生物学、电生理学等的飞速发展，应激研究领域正在向分子水平深入和整体网络水平延伸。转基因技术、基因敲除模型、蛋白缺失实验等技术的发展与广泛应用，为应激机制的研究提供了新思路与新手段。

关键词: 应激, 动物模型, 鼠, 评价, 综述文献

Abstract:

BACKGROUND: People are paying close attention to the relationship between stress and disease. Establishment of animal stress models is the basic for stress research, which is lack of united standard. 
OBJECTIVE: To introduce animal model construction methods and effect evaluation, in addition, to analyze existing problems during model preparation.
METHODS: A computer-based online search was performed using key words of “stress, animal model, murine, and evaluate” for documents on VIP and PuMed from 2000 to 2010. Repetitive papers were excluded. 
RESULTS AND CONCLUSION: A total of 417 documents were collected, including 115 in Chinese and 302 in English. By reading titles and abstract, 30 papers were included in the final analysis. Currently, various animal stress models are involved, but there is not a unified standard, and the stress degree remains lack of quantizatable index. With rapidly development of neuroscience, molecular biology, and electrophysiology, the stress research is extended into molecular level and integrated network. The wide application of transgenic technology, gene knockout models, and protein depletion provide new approach for study of stress mechanism.

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