中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2038-2041.doi: 10.3969/j.issn.1673-8225.2011.11.034

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

金匮肾气丸对肾阳虚证雄性大鼠生精细胞中凋亡相关蛋白Bcl-2、Bax的影响

刘贺亮1，陈长生2，秦  军1，杨  波1，秦卫军1，张  更1，袁建林1   

  1. 1解放军第四军医大学西京医院泌尿外科，陕西省西安市  710033
    2解放军第四军医大学军事预防医学系卫生统计学教研室，陕西省西安市  710033
  • 收稿日期:2010-09-16 修回日期:2010-10-30 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 陈长生，博士，教授，解放军第四军医大学军事预防医学系卫生统计学教研室，陕西省西安市 710033 chencs@fmmu.edu.cn 袁建林，博士，教授，解放军第四军医大学西京医院泌尿外科，陕西省西安市 710033 ccscbr@sina.com
  • 作者简介:刘贺亮☆，男，1966年生，天津市蓟县人，汉族，1998年解放军第四军医大学毕业，博士，副主任医师，副教授，主要从事泌尿系疾病中医治疗、男性不育、泌尿系肿瘤、前列腺疾病等方面的研究。 liuhel@fmmu.edu.cn
  • 基金资助:

    国家自然科学基金(30772683)；陕西省科技计划攻关项目(2008K04-02)。

Effect of Jingguishenqi pill on expression of apoptosis-related protein Bcl-2 and Bax in male mouse with kidney yang deficiency

Liu He-liang1, Chen Chang-sheng2, Qin Jun1, Yang Bo1, Qin Wei-jun1, Zhang Geng1, Yuan Jian-lin1   

  1. 1Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi’an  710033, Shaanxi Province, China
    2Room of Military Preventive Medicine, Department of Health Statistics, Fourth Military Medical University, Xi’an  710033, Shaanxi Province, China
  • Received:2010-09-16 Revised:2010-10-30 Online:2011-03-12 Published:2011-03-12
  • Contact: Chen Chang-sheng, Doctor, Professor, Room of Military Preventive Medicine, Department of Health Statistics, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China chencs@fmmu.edu.cn Yuan Jian-lin, Doctor, Professor, Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China ccscbr@sina.com
  • About author:Liu He-liang☆, Doctor, Associate chief physician, Associate professor, Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China liuhel@fmmu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30772683*; the Tackle Key Program in Science and Technology of Shaanxi Province, No. 2008K04-02*

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摘要：

背景：肾虚可导致不育，金匮肾气丸是治疗肾阳虚证的经典药物，但其作用机制尚不明确。
目的：观察金匮肾气丸对凋亡相关蛋白Bcl-2、Bax在肾阳虚证雄性大鼠生精细胞中表达的影响。
方法：成年雄性SD大鼠100只随机分成5组，采用氢化可的松注射液制备肾阳虚模型。金匮肾气丸低、中、高剂量组在造模基础上每天灌胃含生药0.625，1.250，2.500 g/kg的肾气丸混悬液，连续30 d。正常组和模型组灌胃等量蒸馏水。检测各组大鼠左侧睾丸质量、血清睾丸酮水平、精子密度、精子活率改变情况。采用免疫组织化学方法检测Bcl-2、Bax蛋白在生精细胞中的表达。
结果与结论：模型组左侧睾丸质量、血清睾丸酮水平、精子密度、精子活率低于正常组、金匮肾气丸中、高剂量组(P < 0.05)。与模型组比较，正常组、金匮肾气丸低、中、高剂量组大鼠生精细胞中Bcl-2蛋白表达显著增高(P < 0.05)，Bax蛋白表达显著降低(P < 0.05)。说明金匮肾气丸可使肾阳虚证雄性大鼠生精细胞中Bcl-2蛋白表达升高，Bax蛋白表达降低，从而抑制细胞凋亡，对肾阳虚证雄性大鼠生殖能力有较好的治疗作用。

关键词: 金匮肾气丸, 凋亡, Bcl-2, Bax, 肾阳虚证模型, 生精功能障碍

Abstract:

BACKGROUND: Kidney-deficiency causes sterility. Jingguishenqi pill is a classical Chinese traditional medicine for treating kidney yang deficiency, but its mechanism remains poorly understood.
OBJECTIVE: To explore the effect of Jingguishenqi pill on expression of Bcl-2 and Bax in male mice permatogenic cells with kidney yang deficiency.
METHODS: Totally 100 mature male rats were divided into 5 groups randomly: blank control group (normal group), model group, Jingguishenqi pill with low dosage group, middle dosage group, high dosage group. Except the normal group, the others were made into model of kidney-yang deficiency by injecting hydrocortisone. After the model preparation, the mice in the normal and model groups were fed with distilled waters, and those in the Jingguishenqi pill with different dosage groups were fed with the same dose of medicine (0.625, 1.250 and 2.500 g/kg) for 30 days continuously. Then, the weight of the left testis was measured, and the blood level of testosterone, and the sperm density, survival rate were examined. Then the expression of Bcl-2 and Bax in spermatogenic cells were investigated with the immunohistochemistry and image analysis.
RESULTS AND CONCLUSION: The weight of left testis, blood level of testosterone, sperm density, sperm survival rate in the model group were decreased than that in the control, Jingguishenqi pill with low dosage group, middle dosage group, high dosage group (P < 0.05). The number of cells of expressing Bcl-2 and the intensity of its expression in the model group were lower than that in other four groups (P < 0.05). The number of cells of expressing Bax and the intensity of its expression in the model group were higher than that in other four groups (P < 0.05). Jingguishenqi pill can promote the expression of Bcl-2 but reduce Bax expression, which exerts a certain therapeutic effect on the kidney-yang deficiency in rats.

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